Anti-Myosin Light Chain 2抗体[AT3B2] (ab89594)

概述

  • 产品名称Anti-Myosin Light Chain 2抗体[AT3B2]
    参阅全部 Myosin Light Chain 2 一抗
  • 描述
    小鼠单克隆抗体[AT3B2] to Myosin Light Chain 2
  • 经测试应用适用于: Flow Cyt, WB, Indirect ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Rabbit, Human
  • 免疫原

    Recombinant full length Human Myosin Light Chain 2 purified from E. coli, amino acids 1-166. (NP_000423)

  • 阳性对照
    • Rat heart extracts.

性能

应用

Our Abpromise guarantee covers the use of ab89594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Indirect ELISA Use at an assay dependent concentration.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 疾病相关Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • 序列相似性Contains 3 EF-hand domains.
  • 翻译后修饰N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all

Anti-Myosin Light Chain 2 antibody [AT3B2] 图像

  • Overlay histogram showing A431 cells stained with ab89594 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89594, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/1000 dilution + rat heart extracts at 60 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • ab98594 staining Myosin Light Chain 2 in Rabbit heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 60 minutes; antigen retrieval was by heat mediation in a Tris/EDTA buffer. Samples were incubated with primary antibody (1/500 in PBS-T) for 1 hour at 37°C. A HRP-conjugated Goat anti-mouse polyclonal (1/1000) was used as the secondary antibody.

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  • Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/2000 dilution + Rabbit heart tissue lysate at 10 µg

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

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Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)参考文献

This product has been referenced in:
  • Beveridge RD  et al. The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling. Cell Cycle 13:3450-9 (2014). Human . Read more (PubMed: 25485589) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (Heart)
Loading amount 10 µg
Specification Heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
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Abcam user community

Verified customer

提交于 Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Heart)
Specification Heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Abcam user community

Verified customer

提交于 Feb 21 2013

Thank you for contacting Abcam regarding ab92721 and ab89594. I have received the following protocol information from the laboratory: Western Blotting Protocol Solutions and Reagents 2X Laemmli Sample Buffer: 62.5 mM Tris-HCl (pH 6.8), ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hESC-derived cardiomyocyte)
Specification hESC-derived cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"