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Recombinant full length Human Myosin Light Chain 2 purified from E. coli, amino acids 1-166. (NP_000423)
Our Abpromise guarantee covers the use of ab89594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|WB||1/1000 - 1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).|
|Indirect ELISA||Use at an assay dependent concentration.|
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
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