Anti-Myosin Light Chain 2抗体(ab48003)
Key features and details
- Rabbit polyclonal to Myosin Light Chain 2
- Suitable for: ICC/IF, WB, ELISA, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Myosin Light Chain 2抗体
参阅全部 Myosin Light Chain 2 一抗 -
描述
兔多克隆抗体to Myosin Light Chain 2 -
宿主
Rabbit -
特异性
ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. -
经测试应用
适用于: ICC/IF, WB, ELISA, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide:
GANSNVFSMFEQTQIQ
, corresponding to amino acids 12/27 of Human Myosin Light Chain 2 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48003于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 23704920
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WB |
1/500 - 1/2000. Predicted molecular weight: 19 kDa.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23704920 |
WB
1/500 - 1/2000. Predicted molecular weight: 19 kDa. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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疾病相关
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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数据库链接
- Entrez Gene: 416874 Chicken
- Entrez Gene: 505519 Cow
- Entrez Gene: 403614 Dog
- Entrez Gene: 4633 Human
- Entrez Gene: 17906 Mouse
- Entrez Gene: 363925 Rat
- Entrez Gene: 677750 Zebrafish
- Omim: 160781 Human
see all -
别名
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
see all
图片
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All lanes : Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution
Lane 1 : Vascular myosin (rat aorta), loaded with 35 µg of lysate
Lane 2 : Cardiac myosin (mouse heart, loaded with 35 µg of lysate
Secondary
All lanes : 1:10,000 dilution of
IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]
Predicted band size: 19 kDa
Observed band size: 19,24 kDa why is the actual band size different from the predicted?
Arrowheads indicate the detection of both monophosphorylated (upper) and unphosphorylated (lower) forms of the protein. -
ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
实验方案
数据表及文件
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Datasheet download
文献 (15)
ab48003 被引用在 15 文献中.
- Manivannan SN et al. Novel frameshift variant in MYL2 reveals molecular differences between dominant and recessive forms of hypertrophic cardiomyopathy. PLoS Genet 16:e1008639 (2020). PubMed: 32453731
- Buikema JW et al. Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes. Cell Stem Cell 27:50-63.e5 (2020). PubMed: 32619518
- Park J et al. Switch-like enhancement of epithelial-mesenchymal transition by YAP through feedback regulation of WT1 and Rho-family GTPases. Nat Commun 10:2797 (2019). PubMed: 31243273
- Collins BC et al. Deletion of estrogen receptor a in skeletal muscle results in impaired contractility in female mice. J Appl Physiol (1985) 124:980-992 (2018). PubMed: 29345963
- Prystopiuk V et al. A two-phase response of endothelial cells to hydrostatic pressure. J Cell Sci 131:N/A (2018). PubMed: 29848657