Anti-Myosin Light Chain 2抗体(ab48003)


  • 产品名称
    Anti-Myosin Light Chain 2抗体
    参阅全部 Myosin Light Chain 2 一抗
  • 描述
    兔多克隆抗体to Myosin Light Chain 2
  • 特异性
    ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.
  • 经测试应用
    适用于: ELISA, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 12/27 of Human Myosin Light Chain 2

  • 阳性对照
    • rat aorta



Our Abpromise guarantee covers the use of ab48003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/500 - 1/2000. Predicted molecular weight: 19 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 23704920
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 疾病相关
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • 序列相似性
    Contains 3 EF-hand domains.
  • 翻译后修饰
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all


  • All lanes : Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution

    Lane 1 : Vascular myosin (rat aorta), loaded with 35 µg of lysate
    Lane 2 : Cardiac myosin (mouse heart, loaded with 35 µg of lysate

    1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]

    Predicted band size : 19 kDa
    Observed band size : 19,24 kDa (why is the actual band size different from the predicted?)
  • ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Patrinostro X  et al. Relative importance of ßcyto- and ?cyto-actin in primary mouse embryonic fibroblasts. Mol Biol Cell 28:771-782 (2017). Read more (PubMed: 28077619) »
  • Lu L  et al. Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3. J Biol Chem 290:4663-76 (2015). Read more (PubMed: 25538241) »

See all 8 Publications for this product


Immunocytochemistry/ Immunofluorescence
Human Cell (Cardiac cells)
Yes - 0.05% TritionX100
Cardiac cells
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Sep 16 2016

We recommend use of a 5% non fat dry milk/TBS blocking and incubation solution. We also recommend a starting dilution for the antibody within the range 1/500 - 1/2000. This is just a suggested starting range for the product. The optimal antibody diluti...

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Thank you for your reply.

I can confirm that the immunogen for ab48003 was a synthetic peptide corresponding to amino acids 12 to 27 of Human Myosin Light Chain 2 (GANSNVFSMFEQTQIQ).

The antibody therefore binds to this region.
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I have issued a free of charge replacement for one vial of ab92721. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abprom...

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DISCOUNT CODE: *** Expiration date: April 6th, 2012 I am very pleased to hear you would like to accept our offer and test ab48003 in ICC on mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please ...

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