概述
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产品名称Anti-Myosin 1C抗体- Carboxyterminal end
参阅全部 Myosin 1C 一抗 -
描述兔多克隆抗体to Myosin 1C - Carboxyterminal end
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经测试应用适用于: WB, IHC-P, ELISAmore details
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种属反应性与反应: Human
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阳性对照
- K562 cell lysate, human prostate carcinoma tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab71486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/50 - 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). | |
| IHC-P | 1/10 - 1/50. | |
| ELISA | 1/1000. |
靶标
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功能Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes.
Isoform 3 is involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation. -
序列相似性Contains 2 IQ domains.
Contains 1 myosin head-like domain. -
结构域Binds directly to large unilamellar vesicles (LUVs) containing phosphatidylinositol 4,5-bisphosphate (PIP2) or inositol 1,4,5-trisphosphate (InsP3). The PIP2-binding site corresponds to a putative PH domain present in its tail domain.
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细胞定位Cytoplasm. Cell membrane. Cell projection > stereocilium membrane. Colocalizes with CABP1 and CIB1 at cell margin, membrane ruffles and punctate regions on the cell membrane. Colocalizes in adipocytes with GLUT4 in actin-based membranes. Localizes transiently at cell membrane to region known to be enriched in PIP2. Activation of phospholipase C results in its redistribution to the cytoplasm and Nucleus > nucleoplasm. Nucleus > nucleolus. Nucleus > nuclear pore complex. Colocalizes with RNA polymerase II in the nucleus. Colocalizes with RNA polymerase I in nucleoli (By similarity). In the nucleolus, is localized predominantly in dense fibrillar component (DFC) and in granular component (GC). Accumulates strongly in DFC and GC during activation of transcription. Colocalizes with transcription sites. Colocalizes in the granular cortex at the periphery of the nucleolus with RPS6. Colocalizes in nucleoplasm with RPS6 and actin that are in contact with RNP particles. Colocalizes with RPS6 at the nuclear pore level.
- Information by UniProt
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数据库链接
- Entrez Gene: 4641 Human
- Omim: 606538 Human
- SwissProt: O00159 Human
- Unigene: 286226 Human
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别名
- MMI beta antibody
- MMI-beta antibody
- MMIb antibody
see all
Anti-Myosin 1C antibody - Carboxyterminal end 图像
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Western blot - Myosin 1C antibody (ab71486)Anti-Myosin 1C antibody - Carboxyterminal end (ab71486) at 1/60 dilution + K562 cell lysate at 35 µg
Predicted band size : 118 kDa
Observed band size : 118 kDa
Additional bands at : multiple kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin 1C antibody (ab71486)Human prostate carcinoma tissue with ab71486 at 1/10 dilution. DAB staining. -
Western blot - Anti-Myosin 1C antibody - Carboxyterminal end (ab71486)All lanes : Anti-Myosin 1C antibody - Carboxyterminal end (ab71486) at 1/1000 dilution
Lane 1 : Human 293T whole cell lysate
Lane 2 : Human 293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 118 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteThis image is courtesy of an anonymous Abreview
Anti-Myosin 1C antibody - Carboxyterminal end (ab71486)参考文献
ab71486 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"