Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MYOM2.
- This antibody gave a positive signal in Human Skeletal Muscle and Human Heart Tissue Lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 174 kDa (predicted molecular weight: 165 kDa).
Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.
Contains 5 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Information by UniProt
- 165 kDa connectin associated protein antibody
- 165 kDa connectin-associated protein antibody
- 165 kDa titin associated protein antibody
Western blot - Anti-MYOM2 antibody (ab93915)
All lanes :
Anti-MYOM2 antibody (ab93915) at 1 µg/mlLane 1 :
Human skeletal muscle tissue lysate - total protein (ab29330
)Lane 2 :
Human heart tissue lysate - total protein (ab29431
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
165 kDaObserved band size:
174 kDa (why is the actual band size different from the predicted?
)Additional bands at:
60 kDa. We are unsure as to the identity of these extra bands.Exposure time:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"