重组Anti-Myoglobin抗体[EP3081Y] (ab77232)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3081Y] to Myoglobin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Myoglobin抗体[EP3081Y]
参阅全部 Myoglobin 一抗 -
描述
兔单克隆抗体[EP3081Y] to Myoglobin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Myoglobin aa 100-200 (C terminal). The exact sequence is proprietary.
Database link: P02144 -
阳性对照
- Human, rat and mouse muscle tissue lysates; human heart muscle tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 1.78 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3081Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77232于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols. |
|
ICC/IF |
Use at an assay dependent concentration.
|
说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
序列相似性
Belongs to the globin family. - Information by UniProt
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数据库链接
- Entrez Gene: 4151 Human
- Entrez Gene: 17189 Mouse
- Entrez Gene: 59108 Rat
- Omim: 160000 Human
- SwissProt: P02144 Human
- SwissProt: P04247 Mouse
- SwissProt: Q9QZ76 Rat
- Unigene: 517586 Human
see all -
别名
- MB antibody
- MGC13548 antibody
- MYG_HUMAN antibody
- Myoglobin antibody
图片
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All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Purified)
Lane 1 : Human muscle lysate with 5% NFDM/TBST
Lane 2 : Mouse muscle lysate with 5% NFDM/TBST
Lane 3 : Rat muscle lysate with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 1 second -
Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
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Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) and DAPI.
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Purified ab77232 staining Myoglobin in Mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
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Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
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Unpurified ab77232 showing positive staining in human Skeletal muscle tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with unpurified ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4ºC. A IRDye® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.
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All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Unpurified)
Lane 1 : Human heart muscle lysate
Lane 2 : Mouse heart muscle lysate
Lane 3 : Rat heart muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab77232 showing negative staining in Normal human brain tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab77232 showing negative staining in Normal human kidney tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab77232 showing negative staining in Normal human liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Produced using unpurified ab77232.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab77232 被引用在 29 文献中.
- Majerczak J et al. Nitrite Concentration in the Striated Muscles Is Reversely Related to Myoglobin and Mitochondrial Proteins Content in Rats. Int J Mol Sci 23:N/A (2022). PubMed: 35269826
- Liu F et al. HDAC9 exacerbates myocardial infarction via inactivating Nrf2 pathways. J Pharm Pharmacol N/A:N/A (2021). PubMed: 33963859
- Blackburn ML et al. On the potential role of globins in brown adipose tissue: a novel conceptual model and studies in myoglobin knockout mice. Am J Physiol Endocrinol Metab 321:E47-E62 (2021). PubMed: 33969705
- Elsherbiny ME et al. Expression of Myoglobin in Normal and Cancer Brain Tissues: Correlation With Hypoxia Markers. Front Oncol 11:590771 (2021). PubMed: 33996536
- Ono-Moore KD et al. Metabolic physiology and skeletal muscle phenotypes in male and female myoglobin knockout mice. Am J Physiol Endocrinol Metab 321:E63-E79 (2021). PubMed: 33969704