重组
RabMAb

Anti-Myoglobin抗体[EP3081Y] (ab77232)

概述

  • 产品名称
    Anti-Myoglobin抗体[EP3081Y]
    参阅全部 Myoglobin 一抗
  • 描述
    兔单克隆抗体[EP3081Y] to Myoglobin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Myoglobin aa 100-200 (C terminal). The exact sequence is proprietary.
    Database link: P02144

  • 阳性对照
    • Human, rat and mouse muscle tissue lysates; human heart muscle tissue.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab77232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP 1/40.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols.

ICC/IF Use at an assay dependent concentration.

靶标

图片

  • Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

  • All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Purified)

    Lane 1 : Human muscle lysate with 5% NFDM/TBST
    Lane 2 : Mouse muscle lysate with 5% NFDM/TBST
    Lane 3 : Rat muscle lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 1 second
  • Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) and DAPI.

  • Purified ab77232 staining Myoglobin in Mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

  • Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

  • Unpurified ab77232 showing positive staining in Skeletal muscle tissue.

  • Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with unpurified ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4ºC. A IRDye® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Unpurified)

    Lane 1 : Human heart muscle lysate
    Lane 2 : Mouse heart muscle lysate
    Lane 3 : Rat heart muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.

  • Unpurified ab77232 showing negative staining in Normal brain tissue.

  • Unpurified ab77232 showing negative staining in Normal kidney tissue.

  • Unpurified ab77232 showing negative staining in Normal liver tissue.

  • Produced using unpurified ab77232.

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

This product has been referenced in:
  • Park C  et al. MR Assessment of Acute Pathologic Process after Myocardial Infarction in a Permanent Ligation Mouse Model: Role of Magnetic Nanoparticle-Contrasted MRI. Contrast Media Mol Imaging 2017:2870802 (2017). Read more (PubMed: 29114174) »
  • Hersrud SL  et al. Plasma biomarkers for neuronal ceroid lipofuscinosis. FEBS J 283:459-71 (2016). Read more (PubMed: 26565144) »

See all 7 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (14% 37.5:1)
Sample
Pig Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Thermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 16 2013

Thank you for contacting us.

The IgG concentration ab77232, lot GR52810, is 0.100 mg/mL.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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