概述
- 产品名称Anti-Myoglobin抗体
参阅全部 Myoglobin 一抗 - 描述兔多克隆抗体to Myoglobin
- 经测试应用适用于: WB, Sandwich ELISAmore details
- 种属反应性与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Myoglobin.
(Peptide available as ab88944.)
- 阳性对照
- This antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle; Human Heart.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Related Products
应用
Our Abpromise guarantee covers the use of ab78441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [BDI927] to Myoglobin (ab19607) as Capture. |
靶标
- 功能Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
- 序列相似性Belongs to the globin family.
- Information by UniProt
-
数据库链接
- Entrez Gene: 4151 Human
- Omim: 160000 Human
- SwissProt: P02145 Chimpanzee
- SwissProt: P02147 Gorilla
- SwissProt: P02144 Human
- Unigene: 517586 Human
-
别名
- MB antibody
- MGC13548 antibody
- MYG_HUMAN antibody
see all
Anti-Myoglobin antibody 图像
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Sandwich ELISA - Myoglobin antibody (ab78441)Standard Curve for Myoglobin (Analyte: Myoglobin protein (ab96036)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI927] to Myoglobin (ab19607) at 5µg/ml and Detector Antibody Rabbit polyclonal to Myoglobin (ab78441) at 0.5µg/ml. -
Western blot - Myoglobin antibody (ab78441)All lanes : Anti-Myoglobin antibody (ab78441) at 0.5 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 5 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 17 kDa
Observed band size : 17 kDa
Exposure time : 10 seconds -
Western blot - Myoglobin protein (ab77876)All lanes : Anti-Myoglobin antibody (ab78441) at 0.5 µg/ml
Lane 1 :Natural Human Myoglobin protein (ab77876) at 0.1 µg
Lane 2 :Natural Human Myoglobin protein (ab77876) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
实验方案
Anti-Myoglobin antibody (ab78441)参考文献
ab78441 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"