兔单克隆抗体[EPR4789] to Myogenin
Mouse, Rat, Human
Synthetic peptide within Human Myogenin. The exact sequence is proprietary.
C2C12 and Human fetal muscle lysates; Human rhabdomyosarcoma tissue.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA, PBS
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
Class C basic helix-loop-helix protein 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)
ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.
Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)
All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 1/1000 dilution Lane 1 : C2C12 lysates Lane 2 : Human fetal muscle lysates Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size: 25 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"