重组
RabMAb

Anti-Myogenin抗体[EPR4789] (ab124800)

概述

  • 产品名称
    Anti-Myogenin抗体[EPR4789]
    参阅全部 Myogenin 一抗
  • 描述
    兔单克隆抗体[EPR4789] to Myogenin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Myogenin. The exact sequence is proprietary.

  • 阳性对照
    • C2C12 and Human fetal muscle lysates; Human rhabdomyosarcoma tissue.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    图片

    • ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.
    • All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 1/1000 dilution

      Lane 1 : C2C12 lysates
      Lane 2 : Human fetal muscle lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    文献

    This product has been referenced in:
    • Akiyama T  et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep 8:1189 (2018). Read more (PubMed: 29352121) »
    • Tang Z  et al. MicroRNA-17-92 Regulates the Transcription Factor E2F3b during Myogenesis In Vitro and In Vivo. Int J Mol Sci 18:N/A (2017). WB . Read more (PubMed: 28362317) »

    See all 10 Publications for this product

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse embryonic stem cells differentiated for 30 d)
    Permeabilization
    Yes - 0.5% Triton
    Specification
    mouse embryonic stem cells differentiated for 30 d
    Blocking step
    PBS+0.1%Triton+ 0.1%BSA + 10% Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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