概述

  • 产品名称
    Anti-Myogenin抗体
    参阅全部 Myogenin 一抗
  • 描述
    兔多克隆抗体to Myogenin
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • 阳性对照
    • Induced culture of E.coli (one shot Top10 competent cells).

性能

应用

Our Abpromise guarantee covers the use of ab82843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/40000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
    • 序列相似性
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bHLHc3 antibody
      • cb553 antibody
      • Class C basic helix-loop-helix protein 3 antibody
      • MYF 4 antibody
      • Myf-4 antibody
      • MYF4 antibody
      • MYOG antibody
      • MYOG_HUMAN antibody
      • Myogenic factor 4 antibody
      • Myogenic factor 4 myogenin antibody
      • Myogenin antibody
      • Myogenin myogenin factor 4 antibody
      • OTTHUMP00000039094 antibody
      see all

    Anti-Myogenin antibody 图像

    • All lanes : Anti-Myogenin antibody (ab82843) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    实验方案

    Anti-Myogenin antibody (ab82843)参考文献

    This product has been referenced in:
    • Little RD  et al. Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. Sci Rep 7:6311 (2017). Read more (PubMed: 28740214) »
    • Kemp CM  et al. The effects of Capn1 gene inactivation on skeletal muscle growth, development, and atrophy, and the compensatory role of other proteolytic systems. J Anim Sci 91:3155-67 (2013). Read more (PubMed: 23798514) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Muscle (gastrocnemius))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    35 µg
    Specification
    Muscle (gastrocnemius)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Ms. Sandra Pérez

    Verified customer

    提交于 Apr 20 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal Muscle)
    Loading amount
    30 µg
    Specification
    Skeletal Muscle
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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