The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/40000 is determined for one of the three peptides.
WB: 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.