Anti-MyoD1 (phospho S200)抗体(ab75909)

概述

  • 产品名称Anti-MyoD1 (phospho S200)抗体
    参阅全部 MyoD1 一抗
  • 描述
    兔多克隆抗体to MyoD1 (phospho S200)
  • 特异性ab75909 detects endogenous levels of MyoD1 only when phosphorylated at serine 200.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human MyoD1 around the phosphorylation site of serine 200 (A-S-SP-P-R).

  • 阳性对照
    • Extracts from HeLa cells treated with vanadate (1mg/ml, 30min).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab75909 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.

靶标

  • 功能Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

Anti-MyoD1 (phospho S200) antibody 图像

  • All lanes : Anti-MyoD1 (phospho S200) antibody (ab75909) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells, un-treated
    Lane 2 : Extracts from HeLa cells treated with vanadate (1mg/ml, 30min)


    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

Anti-MyoD1 (phospho S200) antibody (ab75909)参考文献

ab75909 has not yet been referenced specifically in any publications.

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