概述

  • 产品名称Anti-MyoD1抗体[5.2F]
    参阅全部 MyoD1 一抗
  • 描述
    小鼠单克隆抗体[5.2F] to MyoD1
  • 特异性This antibody does not cross react with Myogenin, Myf5, or Myf6.
  • 经测试应用适用于: ICC/IF, Sandwich ELISA, ELISA, IP, IHC-P, IHC-Fr, Electron Microscopy, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Sheep, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse MyoD1 aa 3-56 (N terminal).
    Sequence:

    LLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRLVH VGAL

  • 阳性对照
    • Rhabdomyosarcoma.

性能

应用

Our Abpromise guarantee covers the use of ab16148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection.
ELISA Use at an assay dependent concentration.
IP Use at 2 µg/mg of lysate.
IHC-P Use a concentration of 2 - 4 µg/ml.
IHC-Fr Use a concentration of 2 - 4 µg/ml.
Electron Microscopy Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.

靶标

Anti-MyoD1 antibody [5.2F] 图像

  • All lanes : Anti-MyoD1 antibody [5.2F] (ab16148) at 1/1000 dilution

    Lane 1 : Mouse muscle tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab16148 staining MyoD1 in rat differentiated skeletal muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde and then blocked using 2% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 8 hours. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical staining using ab16148  at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.

  • Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.

实验方案

Anti-MyoD1 antibody [5.2F] (ab16148)参考文献

This product has been referenced in:
  • Sjöqvist S  et al. Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats. Nat Commun 5:3562 (2014). IHC-Fr ; Rat . Read more (PubMed: 24736316) »
  • Aksoy I  et al. Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells. Nat Commun 5:3719 (2014). Mouse . Read more (PubMed: 24770696) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification Muscle
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Sheep Cell (myoblast/other muscle precursor)
Permeabilization No
Specification myoblast/other muscle precursor
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative Methanol
Username

Abcam user community

Verified customer

提交于 Aug 31 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (Muscle of leg)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Cytrate
Permeabilization No
Specification Muscle of leg
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

提交于 Jul 12 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Myocyte of blood vessel)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Cytrate
Permeabilization No
Specification Myocyte of blood vessel
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

提交于 Jul 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (muscle, satellite cell)
Permeabilization No
Gating Strategy negative control (cells incubated with normal serum instead of primary antibody)
Specification muscle, satellite cell
Preparation Cell harvesting/tissue preparation method: cells detached with HyQtase, 10%FBS/DPBS added, after centrifugation pellet resuspended in 1mM EDTA/PBS, fixed in methanol, washed with sample buffer
Sample buffer: 5 mM EDTA, 1% BSA in DPBS
Fixation Methanol
Username

Abcam user community

Verified customer

提交于 Jan 28 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (muscle, satellite cell)
Permeabilization No
Specification muscle, satellite cell
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 28 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Fish Tissue sections (Skeletal muscle fish of Pseudoplatystoma fasciatum)
Antigen retrieval step None
Permeabilization No
Specification Skeletal muscle fish of Pseudoplatystoma fasciatum
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Acetone
Username

Dr. Danilo Aguiar

Verified customer

提交于 May 27 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 31 2014

I believe that you are referring to the data in rat cells sent to us from one of our customers(http://www.abcam.com/MyoD1-antibody-52F-ab16148/reviews/6194)

As Abcam has not produced this image ourselves we do not have any additional informat...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (C2C12 cell line)
Negative control Rabbit IgG
Specification C2C12 cell line
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 11 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde (final concent
Positive control Bmal1 Ab and A MyoD1 Ab from other company
Username

Abcam user community

Verified customer

提交于 Aug 06 2014

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"