Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Contains 1 basic helix-loop-helix (bHLH) domain.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function. Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Immunocytochemistry/ Immunofluorescence - Anti-MyoD1 antibody [5.2F] (ab16148)Image courtesy of an Abreview submitted by Mal Niladri.
ab16148 staining MyoD1 in rat differentiated skeletal muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde and then blocked using 2% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 8 hours. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
Miersch C et al. Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation. BMC Cell Biol19:2 (2018).
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