Anti-Myocardin抗体(ab107301)

概述

  • 产品名称Anti-Myocardin抗体
    参阅全部 Myocardin 一抗
  • 描述
    兔多克隆抗体to Myocardin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Mouse, Rat, Rabbit, Pig, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Myocardin.

  • 阳性对照
    • This antibody gave a positive signal in Human Small Intestine tissue lysate and Caco2 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab107301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).

靶标

  • 功能Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • 组织特异性Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • 序列相似性Contains 3 RPEL repeats.
    Contains 1 SAP domain.
  • 结构域The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

Anti-Myocardin antibody 图像

  • All lanes : Anti-Myocardin antibody (ab107301) at 1 µg/ml

    Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 101 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,26 kDa,48 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-Myocardin antibody (ab107301)参考文献

ab107301 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (heart)
Specification heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Sample Chicken Tissue lysate - whole (Chicken adult heart)
Specification Chicken adult heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Nidia Oliveira

Verified customer

提交于 Aug 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"