Anti-Myocardin抗体(ab106346)

概述

  • 产品名称
    Anti-Myocardin抗体
    参阅全部 Myocardin 一抗
  • 描述
    兔多克隆抗体to Myocardin
  • 经测试应用
    适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal domain of Human Myocardin.

性能

应用

Our Abpromise guarantee covers the use of ab106346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-Fr 1/50 - 1/500.

靶标

  • 功能
    Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • 组织特异性
    Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • 序列相似性
    Contains 3 RPEL repeats.
    Contains 1 SAP domain.
  • 结构域
    The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

文献

ab106346 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (retina)
Antigen retrieval step
None
Permeabilization
Yes - 1% Triton
Specification
retina
Blocking step
normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (pulmonary artery smooth muscle cell)
Loading amount
14 µg
Specification
pulmonary artery smooth muscle cell
Treatment
both treated and not treated by anti-mir145 30nM for 4 days
Gel Running Conditions
Non-reduced Denaturing (10% BisTris Gel)
Blocking step
Licor Blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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