Anti-Myocardin抗体(ab106346)

概述

  • 产品名称Anti-Myocardin抗体
    参阅全部 Myocardin 一抗
  • 描述
    兔多克隆抗体to Myocardin
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal domain of Human Myocardin.

性能

应用

Our Abpromise guarantee covers the use of ab106346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-Fr 1/50 - 1/500.

靶标

  • 功能Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • 组织特异性Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • 序列相似性Contains 3 RPEL repeats.
    Contains 1 SAP domain.
  • 结构域The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

Anti-Myocardin antibody (ab106346)参考文献

ab106346 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (pulmonary artery smooth muscle cell)
Loading amount 14 µg
Specification pulmonary artery smooth muscle cell
Treatment both treated and not treated by anti-mir145 30nM for 4 days
Gel Running Conditions Non-reduced Denaturing (10% BisTris Gel)
Blocking step Licor Blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C
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提交于 Sep 15 2011

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