概述

  • 产品名称Anti-MYO6抗体
    参阅全部 MYO6 一抗
  • 描述
    兔多克隆抗体to MYO6
  • 经测试应用适用于: IHC-P, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to an epitope mapping within the carboxy-terminal part of human Myosin VI with N-terminal added cysteine, conjugated to maleimide activated KLH.

  • 阳性对照
    • MDCK whole cell lysate (immunoblotting), NRK whole cell lysate (immunocytochemistry).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 25232514
ICC 1/75.
WB 1/1000. Detects a band of approximately 150 kDa.

靶标

  • 功能Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells.
  • 组织特异性Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells.
  • 疾病相关Defects in MYO6 are the cause of deafness autosomal dominant type 22 (DFNA22) [MIM:606346]. DFNA22 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA22 is progressive and postlingual, with onset during childhood. By the age of approximately 50 years, affected individuals invariably have profound sensorineural deafness.
    Defects in MYO6 are the cause of deafness autosomal recessive type 37 (DFNB37) [MIM:607821].
    Defects in MYO6 are the cause of deafness sensorineural with hypertrophic cardiomyopathy (DFNHCM) [MIM:606346].
  • 序列相似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 结构域Divided into three regions: a N-terminal motor (head) domain, followed by a neck domain consisting of a calmodulin-binding linker domain and a single IQ motif, and a C-terminal tail region with a coiled-coil and a unique globular domain required for interaction with other proteins.
  • 翻译后修饰Phosphorylation in the motor domain, induced by EGF, results in translocation of MYO6 from the cell surface to membrane ruffles and affects F-actin dynamics. Phosphorylated in vitro by p21-activated kinase (PAK).
  • 细胞定位Cytoplasmic vesicle > clathrin-coated vesicle membrane; Cytoplasmic vesicle > clathrin-coated vesicle membrane. Cell projection > ruffle membrane and Golgi apparatus > trans-Golgi network membrane. Golgi apparatus. Nucleus. Cytoplasm > perinuclear region. Membrane > clathrin-coated pit. Cell projection > ruffle membrane. Also present in endocyctic vesicles, and membrane ruffles. Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage. Recruited into membrane ruffles from cell surface by EGF-stimulation. Colocalizes with DAB2 in clathrin-coated pits/vesicles. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deafness autosomal recessive 37 antibody
    • DFNA 22 antibody
    • DFNA22 antibody
    • DFNB 37 antibody
    • DFNB37 antibody
    • KIAA0389 antibody
    • MYO 6 antibody
    • Myo6 antibody
    • MYO6_HUMAN antibody
    • Myosin VI antibody
    • Myosin-VI antibody
    • Myosin6 antibody
    • Unconventional myosin-6 antibody
    • Unconventional myosin-VI antibody
    see all

Anti-MYO6 antibody 图像

  • ab11096 staining Myo6 in Vero cell line from African green monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence).

Anti-MYO6 antibody (ab11096)参考文献

This product has been referenced in:
  • Nakamura M  et al. Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease. Am J Neurodegener Dis 3:93-102 (2014). IHC-P ; Human . Read more (PubMed: 25232514) »
  • Emmott E  et al. Quantitative proteomics using stable isotope labeling with amino acids in cell culture reveals changes in the cytoplasmic, nuclear, and nucleolar proteomes in vero cells infected with the coronavirus infectious bronchitis virus. Mol Cell Proteomics 9:1920-36 (2010). ICC/IF ; African Green Monkey . Read more (PubMed: 20467043) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (endothelial)
Specification endothelial
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.1%
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Laurence Walch

Verified customer

提交于 Jun 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Arterial endothelial cells)
Loading amount 30 µg
Specification Arterial endothelial cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Elena Astanina

Verified customer

提交于 Apr 09 2008

Thank you for your phone call. The immunogen used was a synthetic peptide corresponding to an epitope mapping within the carboxy-terminal part of human Myosin VI with N-terminal added cysteine, conjugated to maleimide activated KLH. If you have any ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"