Anti-MYO5B抗体(ab58766)

概述

  • 产品名称Anti-MYO5B抗体
    参阅全部 MYO5B 一抗
  • 描述
    兔多克隆抗体to MYO5B
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to N-terminal residues of human MYO5B.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 214 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.
  • 疾病相关Diarrhea 2, with microvillus atrophy
  • 序列相似性Contains 1 dilute domain.
    Contains 6 IQ domains.
    Contains 1 myosin head-like domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1119 antibody
    • Myo5b antibody
    • MYO5B_HUMAN antibody
    • Myosin Vb antibody
    • Unconventional myosin-Vb antibody
    see all

Anti-MYO5B antibody 图像

  • ab58766 staining MYO5B in mouse hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% saponin) for 20 hours at 4°C. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

Anti-MYO5B antibody (ab58766)参考文献

ab58766 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Hippocampal neurons)
Specification Hippocampal neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Mar 17 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (7% SDS)
Sample Mouse Tissue lysate - other (brain)
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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提交于 Mar 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"