概述

  • 产品名称Anti-MYO18A抗体
    参阅全部 MYO18A 一抗
  • 描述
    兔多克隆抗体to MYO18A
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 300-350 of human MYO18A (NP_510880.2).

  • 阳性对照
    • RIPA whole cell lysate from HeLa cells.
  • 常规说明


    ab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab81689 was affinity purified using an epitope specific to MYO18A immobilized on solid support.
  • Primary antibody说明ab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab81689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性Myo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE) rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid associated lung collectin that mediates innate and adaptive immune responses in the lungs.
    • 细胞定位Isoform 1: Endoplasmic reticulum Golgi intermediate compartment. Cytoplasm, cytoskeleton. Note: Colocalizes with actin. Isoform 2: Cytoplasm. Note: Lacks the PDZ domain. Diffusely localized in the cytoplasm.
    • 数据库链接
    • 别名
      • MAJN antibody
      • Molecule associated with JAK3 N terminus antibody
      • Myosin 18A antibody
      • Myosin containing a PDZ domain antibody
      • Myosin XVIIIA antibody
      • MyoXVIIIIA antibody
      • MYSPDZ antibody
      • SPR210 antibody
      see all

    Anti-MYO18A antibody 图像

    • Detection of human MYO18A by Western Blot of Immunoprecipitates.
      Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
      ab81689 used for IP at 3 µg/mg lysate (lane 1).
      MYO18A was also immunoprecipitated using anti MYO18A (ab81690), which recognizes a downstream epitope (lane 2).
      Lane 3 represents the control IgG.
      For blotting immunoprecipitates, ab81689 was used at 1 µg/ml.
      Detection: by Chemiluminescence with an exposure time of 1 second.

    Anti-MYO18A antibody (ab81689)参考文献

    ab81689 has not yet been referenced specifically in any publications.

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