antigen sequence corresponding to amino acids 27-144 of Human MYLK3. (Q32MK0).
- Human gall bladder tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in smooth muscle contraction.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Information by UniProt
- caMLCK antibody
- cardiac MLCK antibody
- Cardiac MyBP C associated Ca/CaM kinase antibody
Immunocytochemistry/ Immunofluorescence - Anti-MYLK3 antibody (ab122643)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122643 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYLK3 antibody (ab122643)
ab122643, at 1/500, staining MYLK3 in paraffin embedded Human gall bladder by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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