Anti-MYLIP抗体(ab107168)

概述

  • 产品名称Anti-MYLIP抗体
    参阅全部 MYLIP 一抗
  • 描述
    兔多克隆抗体to MYLIP
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MYLIP.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; SHSY5Y; NIH3T3.

性能

应用

Our Abpromise guarantee covers the use of ab107168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • 组织特异性Ubiquitously expressed.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 FERM domain.
    Contains 1 RING-type zinc finger.
  • 发展阶段Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.
  • 结构域The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
  • 翻译后修饰Autoubiquitinated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BM-023 antibody
    • BZF1 antibody
    • E3 ubiquitin protein ligase MYLIP antibody
    • E3 ubiquitin-protein ligase MYLIP antibody
    • Idol antibody
    • Inducible degrader of the LDL-receptor antibody
    • Lipoprotein receptor antibody
    • MIR antibody
    • MYLIP antibody
    • MYLIP_HUMAN antibody
    • Myosin regulatory light chain interacting protein antibody
    • PP5242 antibody
    see all

Anti-MYLIP antibody 图像

  • All lanes : Anti-MYLIP antibody (ab107168) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-MYLIP antibody (ab107168)参考文献

ab107168 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview of ab107168. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

Read More
Application Western blot
Sample Mouse Tissue lysate - whole (Left ventricle)
Loading amount 20 µg
Specification Left ventricle
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 29 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Left ventricle, liver, spleen, brain)
Loading amount 20 µg
Specification Left ventricle, liver, spleen, brain
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"