功能Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
组织特异性Smooth muscle tissues and in some, but not all, nonmuscle cells.
序列相似性Contains 3 EF-hand domains.
翻译后修饰Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
ab64161 at 2.5µg/ml staining MYL9 in Colon, Smooth Muscle by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
Western blot - Anti-MYL9 antibody (ab64161)
Anti-MYL9 antibody (ab64161) at 1/2000 dilution + Recombinant Human MYL9 protein (ab104014) at 0.1 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
Anti-MYL9 antibody (ab64161)参考文献
This product has been referenced in:
Hudson CA et al. Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1). Mol Hum Reprod18:265-79 (2012).
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