Myosin light chain 6 alkali smooth muscle and non muscle antibody
Myosin light chain A3 antibody
Myosin light chain alkali 3 antibody
Myosin light polypeptide 6 alkali smooth muscle and non muscle antibody
Myosin light polypeptide 6 antibody
Smooth muscle and non muscle myosin alkali light chain antibody
Smooth muscle and nonmuscle myosin light chain alkali 6 antibody
Western blot - MYL6 antibody (ab84349)
Anti-MYL6 antibody (ab84349) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 17 kDa Observed band size : 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL6 antibody (ab84349)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom.
IHC-P image of MYL6 antibody (ab84349) on seminal vesicle tissue. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.
ICC/IF image of ab84349 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84349, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.