Anti-MYH4抗体(ab111442)

概述

  • 产品名称
    Anti-MYH4抗体
  • 描述
    兔多克隆抗体to MYH4
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from Human MYH4 at C-terminal

  • 阳性对照
    • Human skeletal muscle tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Muscle contraction.
  • 序列相似性
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 结构域
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 细胞定位
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • fetal antibody
    • MYH2B antibody
    • Myh4 antibody
    • MYH4_HUMAN antibody
    • MyHC-2b antibody
    • MyHC-IIb antibody
    • Myosin heavy chain 2b antibody
    • Myosin heavy chain 4 antibody
    • Myosin heavy chain 4 skeletal muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIb antibody
    • Myosin heavy chain skeletal muscle fetal antibody
    • Myosin-4 antibody
    • skeletal muscle antibody
    see all

图片

  • ab111442, at 1/50 dilution, staining MYH4 in paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

文献

ab111442 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Pig Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 500µg/mL · Temperature: 34°C
Username

Md Shamimul Hasan

Verified customer

提交于 Jul 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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