重组
RabMAb

Anti-Myf5抗体[EPR4899] (ab125078)

概述

  • 产品名称
    Anti-Myf5抗体[EPR4899]
    参阅全部 Myf5 一抗
  • 描述
    兔单克隆抗体[EPR4899] to Myf5
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myf5 aa 200 to the C-terminus. The exact sequence is proprietary.

  • 阳性对照
    • WB: Human skeletal muscle, rat muscle, mouse muscle and rat heart tissue lysate.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab125078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa).
IP Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    图片

    • Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified) + Human skeletal muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 28 kDa
      Observed band size: 39 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST
    • All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified)

      Lane 1 : Rat muscle tissue lysate
      Lane 2 : Mouse muscle tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 28 kDa
      Observed band size: 39 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST
    • Ab125078 (Purified) at 1:1000 dilution (0.666 µg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
      Lane 1 (input): Human skeletal muscle
      Lane 2 (+): 125078 & Human skeletal muscle lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM /TBST.

    • All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution (unpurified)

      Lane 1 : Human skeletal muscle tissue lysate
      Lane 2 : Rat muscle tissue lysate
      Lane 3 : Mouse muscle tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 28 kDa

    文献

    This product has been referenced in:
    • Llewellyn KJ  et al. Myogenic differentiation of VCP disease-induced pluripotent stem cells: A novel platform for drug discovery. PLoS One 12:e0176919 (2017). WB ; Human . Read more (PubMed: 28575052) »
    • Tenente IM  et al. Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma. Elife 6:N/A (2017). WB, ICC/IF . Read more (PubMed: 28080960) »

    See all 3 Publications for this product

    客户评价及客户问答

    Thank you for contacting us today.

    As discussed, we do not have a Myf5 antibody tested in ICC and flow cytometry. However, you will be able to get this product for free with any primary antibody purchase. Just quote the code RABMAB-XBSMG wit...

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    Gracias por tu respuesta.

    Si estas interesada en usar los dos anticuerpos propuestos para citometría, ten en cuenta que el anti-Myf5 no se ha testado en esta técnica, y por tanto no podemos asegurar que baya a funcionar. Por eso...

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    Gracias por contactarnos.



    He llevado a cabo una búsqueda en nuestro catálogo de los anticuerpos solicitados. Contra PAX7 tenemos un anticuerpo, creado en conejo y monoclonal, que se ha testado en ratones por Citometr&i...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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