Detects endogenous levels of total Myf5 protein. Cross-reactivity with Myf5, MyoD and Myf6 (greater than 85% sequence homology).
Synthesized peptide derived from an internal sequence of human Myf5.
- Extracts from HepG2 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity purified from rabbit antiserum by affinity chromatography, using
epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
- bHLHc2 antibody
- Class C basic helix loop helix protein 2 antibody
- Class C basic helix-loop-helix protein 2 antibody
Western blot - Myf5 antibody (ab69997)
All lanes : Anti-Myf5 antibody (ab69997) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells, minus immunising peptide
Lane 2 : Extracts from HepG2 cells, plus immunising peptide at 5µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 28 kDa
Observed band size : 28 kDa
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