概述

  • 产品名称
  • 描述
    兔多克隆抗体to Myf5
  • 特异性
    Detects endogenous levels of total Myf5 protein. Cross-reactivity with Myf5, MyoD and Myf6 (greater than 85% sequence homology).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized peptide derived from an internal sequence of human Myf5.

  • 阳性对照
    • Extracts from HepG2 cells

性能

应用

Our Abpromise guarantee covers the use of ab69997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ELISA 1/40000.

靶标

Anti-Myf5 antibody 图像

  • All lanes : Anti-Myf5 antibody (ab69997) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, minus immunising peptide
    Lane 2 : Extracts from HepG2 cells, plus immunising peptide at 5µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

实验方案

Anti-Myf5 antibody (ab69997)参考文献

This product has been referenced in:
  • Bareja A  et al. Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. PLoS One 9:e90398 (2014). ICC ; Mouse, Human . Read more (PubMed: 24587351) »
  • Koch AJ & Holaska JM Loss of Emerin Alters Myogenic Signaling and miRNA Expression in Mouse Myogenic Progenitors. PLoS One 7:e37262 (2012). WB . Read more (PubMed: 22606356) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
35 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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