概述

  • 产品名称Anti-Myf5抗体
    参阅全部 Myf5 一抗
  • 描述
    兔多克隆抗体to Myf5
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide within Human Myf5 aa 200-300. The exact sequence is proprietary.

  • 阳性对照
    • This antibody gave a positive signal in Human Fetal Skeletal Muscle and Mouse E10 Whole Embryo tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab125301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24699686
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

靶标

Anti-Myf5 antibody 图像

  • All lanes : Anti-Myf5 antibody (ab125301) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - Fetal Tissue
    Lane 2 : Mouse Embryo E10 Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 46 kDa,52 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Myf5 antibody (ab125301)参考文献

This product has been referenced in:
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC/IF ; Goat . Read more (PubMed: 24699686) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (muscle, satellite cell)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification muscle, satellite cell
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Brown adipose tissue)
Gel Running Conditions Non-reduced Denaturing (4-12% Bis-Tris MOPS buffer, extended run (60 mins at 180v))
Loading amount 25 µg
Specification Brown adipose tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Miss. Alice Pollard

Verified customer

提交于 May 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"