Myeloperoxidase (MPO) Activity Assay试剂盒(Fluorometric) (ab111749)

Abreviews (1)Q&A (14)
Customer reviews and FAQs

概述

  • 产品名称Myeloperoxidase (MPO) Activity Assay试剂盒(Fluorometric)
    参阅全部 Myeloperoxidase 试剂盒
  • 样品类型
    Cell culture supernatant, Tissue Extracts
  • 检测类型Enzyme activity
  • 灵敏度
    > 0.001 mU/well
  • 种属反应性
    与反应: Mammal
  • 产品概述

    Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) provides a rapid, simple and reliable method to study MPO activity. MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO will react stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm. This kit can be used to detect MPO activity as low as 0.5 µU per well. This kit is suitable for high throughput assay of MPO activity.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 功能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 别名
    • 84 kDa myeloperoxidase
    • 89 kDa myeloperoxidase
    • EC 1.11.1.7
    • EC1.11.2.2
    • fj80f04
    • MPO
    • mpx
    • myeloid-specific peroxidase
    • Myeloperoxidase
    • Myeloperoxidase heavy chain
    • Myeloperoxidase light chain
    • PERM_HUMAN
    • wu:fj80f04
    see all

应用

Our Abpromise guarantee covers the use of ab111749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) 图像

  • MPO activity - ab111749
    MPO activity - ab111749

    a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.

  • Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
    Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
    Standard Curve using this kit protocol (Ex/Em = 485/525 nm)

实验方案

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)参考文献

ab111749 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab111749？请让我们知道，以便我们可以引用本数据表中的参考文章。

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15 Abreviews or Q&A

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MPO activity of ovine lung tissue

 Good Good 4/5 (Ease of Use)
Abreviews
Application: ELISA - MPO fluorometric assay
Samples: 10mg of homogenized lung tissue in 400uL of assay buffer
Loading amount: NEAT
Followed assay protocol to determine neutrophil infiltration in sheep lung tissue.
Standard curve: very high readings, out of range of neat samples - considered diluting standard to get within reference range
Positive control: was at the bottom end of the standard curve and used as a reference only to check the assay was working correctly
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Ms. Gabriela Simonova

Verified customer

提交于 Sep 03 2014

I have 20 different samples that I want to compare the activity for, so I need to normalize the amount of each sample I'm inputting. Would the assay buffer be compatible with a standard protein concentration measurement (eg. BCA assay)?

The laboratory confirmed that the assay buffer will be compatible with a BCA assay, and this method of normalizing your samples' protein concentration would be appropriate in order to make activity level comparisons between your samples.

We are interested in the fluometric mpo activity assay (ab1117490). We were wondering whether it is it advisable to freeze the tissue from which the mpo activity will be analysed? If yes, do you freeze the tissue directly or do you first homogenize and...

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For peroxidase, fresh samples are the best. If needed the tissue can be quick-frozen in liquid nitrogen and stored at -80C. I would not advise making the lysate and then storing it for best results.

Hi,
I will be using your product ab111749 MPO activity assay kit for mouse organ samples.
Since we have many animals to sacrifice, we wonder if we can keep organ samples in freezer for using this MPO activity kit.
I am considering to nec...

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Thank you for your enquiry regarding ab111749.

We have always used with fresh samples only with this assay. We know of scientists who did store the homogenates in aliquots at -80°C for a short while before use but since we don’t our...

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Customer kindly contacted us regarding MPO activity assay kit (Fluorometric) with a couple questions.
1. Can tissue samples homogenized in assay buffer be frozen at -20 or -80? If so, what buffer conditions are appropriate (protease inhibitors, etc...

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The homogenized samples can be stored in aliquots at -80°C, w/o addition of any other components to the assay buffer. It would be preferable though to use fresh samples.
“V” is the volume of the sample you add to the wells in step ...

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‘Can this MPO assay kit (http://www.abcam.com/Myeloperoxidase-MPO-Activity-Assay-Kit-Fluorometric-ab111749.html) be used for rat tissue homogenates, or is it only for human samples?

This kit can be used with all mammalian samples including rats.

I have updated our datasheet accordingly.

How will EDTA inhibit this assay?

This is an enzymatic activity assay. Enzymes need some ions/cofactors to function and EDTA being a chelator will chelate these co-factors (specifically Mg++ and Ca++), leading to the enzyme becoming non-functional.

What is the concentration of the MPO positive control for my lot?

Thank you for your inquiry and your patience with this reply.

I heard back from the supplying laboratory that the concentration of the positive control MPO does not vary from lot to lot. But unfortunately, they could not disclose the concent...

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Should a black plate with clear bottom wells or opaque bottom wells be used with this kit?


For this assay kit, the client can either use a black opaque bottom plate or, for even more efficiency, use a white opaque bottom plate. One would not use a clear transparent plate.

Should a black plate with clear bottom wells or opaque bottom wells be used with this kit?



I am awaiting a reply from the lab which supplies this kit. However I believe you can use a plate with either clear well bottoms or opaque well bottoms to detect fluorometric signal. Although you would use a clear bottom well if you need to v...

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