Myeloperoxidase (MPO) Activity Assay试剂盒(Colorimetric) (ab105136)

概述

  • 产品名称Myeloperoxidase (MPO) Activity Assay试剂盒(Colorimetric)
    参阅全部 Myeloperoxidase 试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • 检测类型Enzyme activity
  • 种属反应性
    与反应: Human
    预测可用于: all Mammals
  • 产品概述

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In ab105136, the HClO produced from H2O2 and Cl- is reacted with taurine to generate the taurine chloramine, which subsequently reacts with the TNB2- probe to eliminate color (lambda = 412 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of Myeloperoxidase. This kit can be used to detect Myeloperoxidase as low as 0.05 mU per well.

  • 说明

    Store the kit at -20 °C protected from light. Allow the Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol prior to performing the assay.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 功能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 别名
    • 84 kDa myeloperoxidase
    • 89 kDa myeloperoxidase
    • EC 1.11.1.7
    • EC1.11.2.2
    • fj80f04
    • MPO
    • mpx
    • myeloid-specific peroxidase
    • Myeloperoxidase
    • Myeloperoxidase heavy chain
    • Myeloperoxidase light chain
    • PERM_HUMAN
    • wu:fj80f04
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab105136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) 图像

  • a. Measurement of MPO activity using WBC lysate (3 μg) and MPO Positive Control (5 and 10 μL). b. MPO specific activity in WBC lysate.

  • Typical standard curve for ab105136.

实验方案

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)参考文献

ab105136 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
I used a different protocol for sample preparation of tissues of mouse origin,. Using the supplied protocol resulted in formation of insoluble material in several dilutions
1) 30 mg of frozen tissue was utilized
2) tissue was once washed in 500 µl PBS followed by centrifugation at 13.000 g for 5 min at 4°C
3) tissue was homogenized in 250 µl PBS containing 10mM NEM (N-Ethylmaleimide) and homogenized with a micro tissue lyzer
4) centrifugation at 13000 g at 4°C for 20 min and the supernatant was removed
5) 250 µl PBS containing 0,5% HTA-Br (w/v) was added
6) Samples were sonicated for 30 sec on ice
7) centrifugation for 20 min at 4°C and 8000g
8) the supernatant was utilized in a dilution of 1:2 to 1:10 for the assay directly
Username

Frau Dr. Katharina Rump

Verified customer

提交于 Mar 01 2016

Higer reading in sample

Inconclusive Average 3/5 (Ease of Use)
Abreviews
I homogenized mouse lever tissues using Bio Masher(nippi) .
First time I used Pestle motor for nippi Bio masher (Left image).
Second Bio Masher using no Pestle motor (Right image).
I can't measure samples OD by automated homoginizer.
First time OD can't be measured diluted with MPO Assay Buffer 1:1000.
Second time OD can be measured at dilution 1:30 (homoginized by hand manual).
Username

眞智子 平賀

Verified customer

提交于 Aug 21 2014

When we measure the positive controls we do it kinetically over time. Then we select a time between 30 mins to 2 hours and make the graph. It is important to note that the activity of the positive control varies from lot to lot and the essence of this ...

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Although it would be preferable to use fresh samples it is probably ok to use frozen serum/plasma with this kit. We ourselves have always used fresh samples with this assay however, we know of scientists who used tissue homogenates stored at -80°C ...

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The composition of the MPO assay buffer is proprietary. However we can share that it is a Citrate-based buffer containing 50 mM Taurine and 0.1% NP-40.

Thank you very much for you call today and for letting us know about the problem with this tube of probe.

As we discussed, please send me the original order or PO number, and I'll see if we can just send you a replacement vial of the probe. <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"