重组Anti-Myeloperoxidase抗体[EPR17996] (ab188211)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17996] to Myeloperoxidase
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Myeloperoxidase抗体[EPR17996]
参阅全部 Myeloperoxidase 一抗 -
描述
兔单克隆抗体[EPR17996] to Myeloperoxidase -
宿主
Rabbit -
特异性
This antibody is specific to Myeloperoxidase light chain. -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and rat spleen lysates. IHC-P: Human, mouse and rat spleen tissues. Flow Cyt (intra): Mouse PBMC, C57 BL/6 mouse bone marrow cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17996 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188211于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/500.
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WB |
1/1000. Detects a band of approximately 89, 74, 13 kDa (predicted molecular weight: 83 kDa).
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IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/500. |
WB
1/1000. Detects a band of approximately 89, 74, 13 kDa (predicted molecular weight: 83 kDa). |
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
疾病相关
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
序列相似性
Belongs to the peroxidase family. XPO subfamily. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 4353 Human
- Entrez Gene: 17523 Mouse
- Entrez Gene: 303413 Rat
- Omim: 606989 Human
- SwissProt: P05164 Human
- SwissProt: P11247 Mouse
- Unigene: 458272 Human
- Unigene: 4668 Mouse
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别名
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
图片
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Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab188211 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30min at 22°C in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab188211 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 0.2 μg/ml (1/10200)) for 30min at 22°C. The cells were simultaneously stained with Ly6G.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of human spleen [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of mouse spleen [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of rat spleen [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Myeloperoxidase antibody [EPR17996] (ab188211) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 13,74,89 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223. 89 kDa (MPO), 74 kDa (intermediate form), 13 kDa (light chain)
Negative control: NIH/3T3 PMID: 9001423.
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Anti-Myeloperoxidase antibody [EPR17996] (ab188211) at 1/1000 dilution + Rat spleen lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 13,74 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature PMID: 2154223.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed mouse PBMC labeling Myeloperoxidase with ab188211 at 1/500 dilution (Right) compared with a rabbit monoclonal IgG isotype control (ab172730; Left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Mouse peripheral blood mononuclear cells stained intracellularly with ab188211 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (8)
ab188211 被引用在 8 文献中.
- Van Mechelen M et al. Impact of barrier tissue inflammation and physical activity on joint homeostasis in mice. Rheumatology (Oxford) 61:1690-1698 (2022). PubMed: 34175921
- Peng J et al. MPO/HOCl Facilitates Apoptosis and Ferroptosis in the SOD1G93A Motor Neuron of Amyotrophic Lateral Sclerosis. Oxid Med Cell Longev 2022:8217663 (2022). PubMed: 35178161
- Beyer AM et al. Autophagy, TERT, and mitochondrial dysfunction in hyperoxia. Am J Physiol Heart Circ Physiol 321:H985-H1003 (2021). PubMed: 34559580
- Yang B et al. Decreased miR-4512 Levels in Monocytes and Macrophages of Individuals With Systemic Lupus Erythematosus Contribute to Innate Immune Activation and Neutrsophil NETosis by Targeting TLR4 and CXCL2. Front Immunol 12:756825 (2021). PubMed: 34721432
- Samain R et al. Pharmacologic Normalization of Pancreatic Cancer-Associated Fibroblast Secretome Impairs Prometastatic Cross-Talk With Macrophages. Cell Mol Gastroenterol Hepatol 11:1405-1436 (2021). PubMed: 33482394
- Zhang SR et al. IL-37 increases in patients after ischemic stroke and protects from inflammatory brain injury, motor impairment and lung infection in mice. Sci Rep 9:6922 (2019). PubMed: 31061403
- Lee JS et al. PCSK9 inhibition as a novel therapeutic target for alcoholic liver disease. Sci Rep 9:17167 (2019). PubMed: 31748600
- Tian X et al. Protective Role of Nuclear Factor-Erythroid 2-Related Factor 2 Against Radiation-Induced Lung Injury and Inflammation. Front Oncol 8:542 (2018). PubMed: 30533397