Anti-Myeloperoxidase抗体(ab9535)

概述

  • 产品名称
    Anti-Myeloperoxidase抗体
    参阅全部 Myeloperoxidase 一抗
  • 描述
    兔多克隆抗体to Myeloperoxidase
  • 经测试应用
    适用于: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig, Monkey
  • 免疫原

    Myeloperoxidase isolated from human granulocytes.

  • 阳性对照
    • Tonsil

性能

应用

Our Abpromise guarantee covers the use of ab9535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25 - 1/50.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性
    Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位
    Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody 图像

  • ab9535 staining Myeloperoxidase in paraffin-embedded rat tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded Immunohistochemistry). Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/50 in milken) for 1 hour ate 24°C. A Biotin-conjugated goat anti rabbt polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • Ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.

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  • Immunohistochemistry (Frozen sections) of Mouse stomach tissue labelling Myeloperoxidase with ab9535 at 5%.

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  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human B Cell Lymphoma tissue, staining Myeloperoxidase with ab9535.

Anti-Myeloperoxidase antibody (ab9535)参考文献

This product has been referenced in:
  • Atwal M  et al. Myeloperoxidase Enhances Etoposide and Mitoxantrone-Mediated DNA Damage: A Target for Myeloprotection in Cancer Chemotherapy. Mol Pharmacol 91:49-57 (2017). ICC/IF . Read more (PubMed: 27974636) »
  • Yu W  et al. A Systems Pharmacology Approach to Determine Active Compounds and Action Mechanisms of Xipayi KuiJie'an enema for Treatment of Ulcerative colitis. Sci Rep 7:1189 (2017). IHC-P ; Mouse . Read more (PubMed: 28446747) »

See all 30 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Mesenteric Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2/ Citrate buffer ph6
Permeabilization
No
Specification
Mesenteric Lymph node
Fixative
Paraformaldehyde
Username

Lina Li

Verified customer

提交于 May 18 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 May 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Normal Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization
No
Specification
Normal Spleen
Blocking step
serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

提交于 Apr 11 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (stomach)
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Mar 10 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Feb 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (liver treated with LPS)
Permeabilization
No
Specification
liver treated with LPS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 14 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (LPS-treated lung)
Permeabilization
No
Specification
LPS-treated lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 Dec 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Blood smear)
Permeabilization
No
Specification
Blood smear
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 Dec 01 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
skin tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Nov 10 2015

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