Anti-Myeloperoxidase抗体(ab9535)

概述

  • 产品名称Anti-Myeloperoxidase抗体
    参阅全部 Myeloperoxidase 一抗
  • 描述
    兔多克隆抗体to Myeloperoxidase
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig, Monkey
  • 免疫原

    Myeloperoxidase isolated from human granulocytes.

  • 阳性对照
    • Tonsil

性能

应用

Our Abpromise guarantee covers the use of ab9535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25 - 1/50.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody 图像

  • ab9535 staining Myeloperoxidase in paraffin-embedded rat tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded Immunohistochemistry). Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/50 in milken) for 1 hour ate 24°C. A Biotin-conjugated goat anti rabbt polyclonal (1/500) was used as secondary antibody.

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  • Ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.

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  • Immunohistochemistry (Frozen sections) of Mouse stomach tissue labelling Myeloperoxidase with ab9535 at 5%.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human B Cell Lymphoma tissue, staining Myeloperoxidase with ab9535.

Anti-Myeloperoxidase antibody (ab9535)参考文献

This product has been referenced in:
  • Ambade A  et al. Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease. World J Gastroenterol 22:4091-108 (2016). IHC-P ; Mouse . Read more (PubMed: 27122661) »
  • Ueno K  et al. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts. Sci Rep 6:28538 (2016). IHC-P ; Mouse . Read more (PubMed: 27329845) »

See all 24 Publications for this product

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 May 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Normal Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization No
Specification Normal Spleen
Blocking step serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
Username

Ms. Premier Laboratory

Verified customer

提交于 Apr 11 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (stomach)
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Mar 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification Bone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Feb 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (liver treated with LPS)
Permeabilization No
Specification liver treated with LPS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 14 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (LPS-treated lung)
Permeabilization No
Specification LPS-treated lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 Dec 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Blood smear)
Permeabilization No
Specification Blood smear
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 Dec 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization No
Specification skin tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Nov 10 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 17 2015

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