Anti-Myeloperoxidase抗体(ab45977)

概述

  • 产品名称Anti-Myeloperoxidase抗体
    参阅全部 Myeloperoxidase 一抗
  • 描述
    兔多克隆抗体to Myeloperoxidase
  • 特异性Ab45977 has been batch tested using human lysates/tissues only. Some customers have successfully used ab45977 with rat. Please contact Abcam Scientific Support for more information.
  • 经测试应用适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Marmoset (common)
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Myeloperoxidase.

    (Peptide available as ab45976.)

  • 阳性对照
    • In Western Blot, ab45977 gave a positive signal in HL60 whole cell lysate. In IHC, this antibody gave a positive signal in Human Tonsil formalin-fixed paraffin embedded tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab45977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).Can be blocked with Myeloperoxidase peptide (ab45976).
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody 图像

  • ICC/IF image of ab45977 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45977, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab45977 (1/50) staining Myeloperoxidase in paraffin-embbeded human spleen tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.

    See Abreview

  • IHC image of 45977 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45977, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunohistochemistical detection of Myeloperoxidase using antibody ab45977 on formaldehyde-fixed paraffin embedded Marmoset spleen sections.  Antigen retrieval step: heat mediated in citric acidpH6 HIER. Permeabilization: No Blocking step: 1% BSA for 10 mins at room temperature. Primary antibody diluted at 1/600 and incubated for 2 hours in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG conjugated to biotin (1/200). Submitted image shows clear scattered cytoplasmic and (possibly secreted) positivity within the red pulp. Also, the blood vessel extending from upper middle to lower left of the image, shows clear, though lighter, granular positivity within the cytoplasm of 5 cells. Taken together, this is the pattern of positivity that I would expect.

    See Abreview

  • ab23106 staining p53 in Mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% Goat serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 9 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.
    Nuclei were counterstained blue with DAPI.

    See Abreview

  • Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa


    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45977 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 12 kDa,35 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    The mature myeloperoxidase protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa).  Our immunogen sequence is within the myeloperoxidase light chain.  In HL60 cells, ab45977 detects bands at approximately 84-kDa, corresponding to the expected MW of full-length Myeloperoxidase protein, and at 12-kDa, corresponding to the expected MW of myeloperoxidase light chain.   

Anti-Myeloperoxidase antibody (ab45977)参考文献

This product has been referenced in:
  • Cao S  et al. Hydrogen sulfide attenuates brain edema in early brain injury after subarachnoid hemorrhage in rats: Possible involvement of MMP-9 induced blood-brain barrier disruption and AQP4 expression. Neurosci Lett 621:88-97 (2016). IHC ; Rat . Read more (PubMed: 27080433) »
  • Shi Y  et al. Rapid endothelial cytoskeletal reorganization enables early blood-brain barrier disruption and long-term ischaemic reperfusion brain injury. Nat Commun 7:10523 (2016). IHC-P ; Mouse . Read more (PubMed: 26813496) »

See all 17 Publications for this product

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Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Spleen)
Specification Spleen
Fixative Acetone
Permeabilization Yes - 0.1%PBS Tween
Blocking step BSA as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Aronaghy

Verified customer

提交于 Oct 24 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cell lysate)
Loading amount 30 µg
Specification Jurkat cell lysate
Gel Running Conditions Reduced Denaturing (8% Gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Dr. G.K Sakellariou

Verified customer

提交于 Oct 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 13 2012

Thank you for contacting us.

These products are sold in liquid form and should not require any reconstitution. When using in your particular assay please dilute in the appropriate buffer for that assay.

I hope this information...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 05 2012

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