Anti-Myeloperoxidase抗体[2C7] (ab25989)

概述

  • 产品名称Anti-Myeloperoxidase抗体[2C7]
    参阅全部 Myeloperoxidase 一抗
  • 描述
    小鼠单克隆抗体[2C7] to Myeloperoxidase
  • 特异性ab25989 recognises myeloperoxidase (MPO).
  • 经测试应用适用于: ICC/IF, IHC-Fr, IHC-P, ELISA, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Myeloperoxidase.

性能

应用

Our Abpromise guarantee covers the use of ab25989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20818377
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 84 kDa.
Flow Cyt 1/20 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody [2C7] 图像

  • Overlay histogram showing HeLa cells stained with ab25989 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25989, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Myeloperoxidase antibody [2C7] (ab25989)参考文献

This product has been referenced in:
  • Silvescu CI & Sackstein R G-CSF induces membrane expression of a myeloperoxidase glycovariant that operates as an E-selectin ligand on human myeloid cells. Proc Natl Acad Sci U S A 111:10696-701 (2014). Human . Read more (PubMed: 25002508) »
  • Douda DN  et al. Akt is essential to induce NADPH-dependent NETosis and to switch the neutrophil death to apoptosis. Blood 123:597-600 (2014). IF . Read more (PubMed: 24458280) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (LIVER)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ph6 (ER1) 20 MINS
Permeabilization No
Specification LIVER
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Christopher Bagnall

Verified customer

提交于 Nov 22 2016

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guara...

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DISCOUNT CODE: xxxxxxx Expiration date: 2012-04-20 As discussed I have generated a discount code for ab25989. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for staining ...

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Thank you for contacting us and considering our testing discount with ab25989. As the sequence of human Myeloperoxidase shares 86% homology with the pig protein we would predict that  ab25989 would be able to recognise this protein. I can issue a te...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (ventricular tissue lysates)
Loading amount 50 µg
Specification ventricular tissue lysates
Gel Running Conditions Non-reduced Denaturing (4~12% gradient NuPAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (human aortic tissue extract)
Loading amount 30 µg
Specification human aortic tissue extract
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Guo-Ping Shi

Verified customer

提交于 Mar 03 2008

Thank you for your enquiry regarding ab25989 and your interest in our products. The immunogen was full-length myeloperoxidase. The antibody recognizes an epitope on the heavy chain, but the exact epitope location has not been mapped.

Ab25989 recognizes an epitope on the heavy chain of myeloperoxidase. The protein is a multimer of 2 heavy chains (55kDa) and two light chains (15kDa), the heavy chains being linked by a disulfide bond. In Western blot under non-reducing conditions, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"