The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
Crook K & Hunt SV Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol4:235-46 (1996).
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