Anti-Myeloid Precursor Cells抗体[ER-MP58] (ab63860)

概述

  • 产品名称
    Anti-Myeloid Precursor Cells抗体[ER-MP58]
  • 描述
    大鼠单克隆抗体[ER-MP58] to Myeloid Precursor Cells
  • 经测试应用
    适用于: IHC-P, IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Balb/c macrophage precursor cell hybrids

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    ER-MP58
  • 骨髓瘤
    Y3/Ag1.2.3
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IP
IHC-Fr
Flow Cyt
  • 应用说明
    Flow Cyt: Use 10µl for 106 cells.
    IHC-P: Use at an assay dependent dilution (PMID 19720836).
    IHC-Fr: 1/25 - 1/100.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      ER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
    • 细胞定位
      Cell Membrane

    图片

    • ab63860 staining Myeloid Precursor Cells in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin. Immunohistochemical analysis was performed with heat-induced antigen retrieval. The primary antibody used was ab63860. A biotinylated secondary antibody was used with and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin. Scale bar, 200 µm.

    文献

    This product has been referenced in:
    • Karpurapu M  et al. Functional PU.1 in macrophages has a pivotal role in NF-?B activation and neutrophilic lung inflammation during endotoxemia. Blood 118:5255-66 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21937699) »
    • Lu X & Kang Y Chemokine (C-C motif) ligand 2 engages CCR2+ stromal cells of monocytic origin to promote breast cancer metastasis to lung and bone. J Biol Chem 284:29087-96 (2009). IHC-P ; Mouse . Read more (PubMed: 19720836) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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