Anti-Myeloid leukemia factor 1抗体(ab70211)

概述

  • 产品名称
    Anti-Myeloid leukemia factor 1抗体
    参阅全部 Myeloid leukemia factor 1 一抗
  • 描述
    兔多克隆抗体to Myeloid leukemia factor 1
  • 特异性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residue 200 and the C-terminus (residue 256) of human Myeloid leukemia factor 1.

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab70211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/5000 - 1/15000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
    • 组织特异性
      Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
    • 疾病相关
      Note=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
    • 序列相似性
      Belongs to the MLF family.
    • 翻译后修饰
      Phosphorylation is required for binding to YWHAZ.
    • 细胞定位
      Cytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Hls7 antibody
      • MLF1 antibody
      • MLF1_HUMAN antibody
      • Myelodysplasia myeloid leukemia factor 1 antibody
      • Myelodysplasia-myeloid leukemia factor 1 antibody
      • Myeloid leukemia factor 1 antibody
      • myeloid leukemia factor 1 variant 1 antibody
      • myeloid leukemia factor 1 variant 2 antibody
      • myeloid leukemia factor 1 variant 3 antibody
      see all

    Anti-Myeloid leukemia factor 1 antibody 图像

    • All lanes : Anti-Myeloid leukemia factor 1 antibody (ab70211) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg


      Predicted band size : 31 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa,250 kDa,28 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human Myeloid leukemia factor 1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded). Affinity purified rabbit anti-Myeloid leukemia factor 1 antibody (ab70211) was used at 3 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for WB detection. Lane 2 represents IP IgG control.

    Anti-Myeloid leukemia factor 1 antibody (ab70211)参考文献

    ab70211 has not yet been referenced specifically in any publications.

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