Anti-Myeloid leukemia factor 1抗体(ab103099)

概述

  • 产品名称Anti-Myeloid leukemia factor 1抗体
    参阅全部 Myeloid leukemia factor 1 一抗
  • 描述
    兔多克隆抗体to Myeloid leukemia factor 1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 222-252 of Human Myeloid leukemia factor 1

  • 阳性对照
    • Mouse heart tissue, Human testis carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab103099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 31 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
  • 组织特异性Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
  • 疾病相关Note=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
  • 序列相似性Belongs to the MLF family.
  • 翻译后修饰Phosphorylation is required for binding to YWHAZ.
  • 细胞定位Cytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hls7 antibody
    • MLF1 antibody
    • MLF1_HUMAN antibody
    • Myelodysplasia myeloid leukemia factor 1 antibody
    • Myelodysplasia-myeloid leukemia factor 1 antibody
    • Myeloid leukemia factor 1 antibody
    • myeloid leukemia factor 1 variant 1 antibody
    • myeloid leukemia factor 1 variant 2 antibody
    • myeloid leukemia factor 1 variant 3 antibody
    see all

Anti-Myeloid leukemia factor 1 antibody 图像

  • Anti-Myeloid leukemia factor 1 antibody (ab103099) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 31 kDa
  • IHC analysis in formalin fixed and paraffin embedded Human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining, using ab103099 at a dilution of 1/50.

Anti-Myeloid leukemia factor 1 antibody (ab103099)参考文献

ab103099 has not yet been referenced specifically in any publications.

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