Anti-Myelin PLP抗体[plpc 1] (ab9311)

概述

  • 产品名称Anti-Myelin PLP抗体[plpc 1]
    参阅全部 Myelin PLP 一抗
  • 描述
    小鼠单克隆抗体[plpc 1] to Myelin PLP
  • 特异性This antibody recognizes myelin proteolipid protein
  • 经测试应用适用于: IHC-FoFr, IHC-Fr, WB, ICC/IF, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide:

    C-GRGTKF

    , corresponding to amino acids 272-277 of Myelin PLP.

性能

应用

Our Abpromise guarantee covers the use of ab9311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 17626200
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/100.
IHC (PFA fixed) 1/1000.

靶标

  • 功能This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • 疾病相关Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • 序列相似性Belongs to the myelin proteolipid protein family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HLD1 antibody
    • Lipophilin antibody
    • Major myelin proteolipid protein antibody
    • MMPL antibody
    • Myelin proteolipid protein antibody
    • MYPR_HUMAN antibody
    • PLP 1 antibody
    • PLP antibody
    • PLP/DM20 antibody
    • PLP1 antibody
    • PMD antibody
    • Proteolipid protein 1 antibody
    • SPG2 antibody
    see all

Anti-Myelin PLP antibody [plpc 1] 图像

  • ab9311 at a dilution of 1/1000, staining neuronal Myelin PLP  (Alexa 488 secondary at 1/2000) in rat brain tissue (30µm thick coronal sections) on free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates

  • ab9311 at 1/100 staining rat spinal cord tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and incubated with the antibody for 24 hours. A Texas Red conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

实验方案

Anti-Myelin PLP antibody [plpc 1] (ab9311)参考文献

This product has been referenced in:
  • Black JA  et al. Nav1.5 sodium channels in macrophages in multiple sclerosis lesions. Mult Scler : (2012). IHC ; Human . Read more (PubMed: 22951351) »
  • Kovalevich J  et al. Cocaine-induced loss of white matter proteins in the adult mouse nucleus accumbens is attenuated by administration of a ß-lactam antibiotic during cocaine withdrawal. Am J Pathol 181:1921-7 (2012). Read more (PubMed: 23031254) »

See all 5 Publications for this product

Product Wall

For western blotting we recommend a starting dilution of 1/1000 and that users titrate from there to determine the optimal concentration for their individual experiments.

Below please find information regarding your order. Please note it FedEx has delivered this package.
Please feel free to contact us if you have any further questions.
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I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

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Thank you for contacting Abcam regarding ab9311.

I am sorry that the customer is experiencing difficulties with this antibody in WB. I have reviewed the protocol information and images and I agree that this antibody is not working as stated ...

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Application Western blot
Sample Rabbit Tissue lysate - whole (Brain)
Loading amount 20 µg
Specification Brain
Gel Running Conditions Reduced Non-Denaturing (Native) (4-15)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Govindaiah Vinukonda

Verified customer

提交于 Jun 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Oligodendroglia)
Specification Oligodendroglia
Fixative Paraformaldehyde
Permeabilization Yes - 0.01% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 07 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (oligodendroglia)
Loading amount 5 µg
Specification oligodendroglia
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2007

Thank you for your enquiry regarding ab9311. This antibody labels myelin PLP protein therefore it will indeed label the myelin rather than the cell body. I would recommend looking at the product ab7474 (in particular the IHC image) which stains for...

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