概述

  • 产品名称
    Anti-Myelin PLP抗体
    参阅全部 Myelin PLP 一抗
  • 描述
    兔多克隆抗体to Myelin PLP
  • 特异性
    ab28486 recognises PLP
  • 经测试应用
    适用于: ELISA, WB, ICC, IHC-FoFr, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Cow, Dog, Pig, Macaque monkey
  • 免疫原

    Synthetic peptide:

    CG KGLSATVTGG QKGRGSRG

    , corresponding to amino acids 109-128 of Mouse Myelin PLP

  • 阳性对照
    • Mouse brain

性能

应用

Our Abpromise guarantee covers the use of ab28486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/1000. Predicted molecular weight: 26,30 kDa.
ICC 1/1000.
IHC-FoFr 1/100.
ICC/IF 1/100.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/500.
Flow Cyt Use at an assay dependent concentration. PubMed: 19659692ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • 疾病相关
    Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • 序列相似性
    Belongs to the myelin proteolipid protein family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HLD1 antibody
    • Lipophilin antibody
    • Major myelin proteolipid protein antibody
    • MMPL antibody
    • Myelin proteolipid protein antibody
    • MYPR_HUMAN antibody
    • PLP 1 antibody
    • PLP antibody
    • PLP/DM20 antibody
    • PLP1 antibody
    • PMD antibody
    • Proteolipid protein 1 antibody
    • SPG2 antibody
    see all

Anti-Myelin PLP antibody 图像

  • ab28486 staining Myelin PLP in Rabbit brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with methanol, permeablized with acetone and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human brain cells, staining Myelin PLP with ab28486.

    Cells were fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/400 in diluent) for 16 hours. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical staining of  Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.

  • ab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.ab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.

    See Abreview

Anti-Myelin PLP antibody (ab28486)参考文献

This product has been referenced in:
  • Labonne JD  et al. A microdeletion at Xq22.2 implicates a glycine receptor GLRA4 involved in intellectual disability, behavioral problems and craniofacial anomalies. BMC Neurol 16:132 (2016). Read more (PubMed: 27506666) »
  • Locatelli G  et al. Mature oligodendrocytes actively increase in vivo cytoskeletal plasticity following CNS damage. J Neuroinflammation 12:62 (2015). Read more (PubMed: 25889302) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Rat Tissue lysate - whole (brain tissue)
Specification
brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Rabbit Tissue sections (brain)
Specification
brain
Permeabilization
Yes - Acetone
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Sep 09 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Indirect
Username

Dr. Rachel Barker

Verified customer

提交于 Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain)
Specification
brain
Fixative
Formaldehyde
Permeabilization
Yes - Citrate
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

提交于 Nov 06 2012

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow.

Please keep me u...

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Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab105784 on the order ***, which should arrive tomorrow. Please keep me updated about ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (C57B/J mouse Vagus Nerve)
Specification
C57B/J mouse Vagus Nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton-X100 in PBS
Blocking step
5% BSA + 0.5% goat serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Mar 01 2012

Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this cas...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Karine Thibault

Verified customer

提交于 Sep 27 2011

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