Anti-Myelin oligodendrocyte glycoprotein抗体(ab115597)

概述

  • 产品名称
    Anti-Myelin oligodendrocyte glycoprotein抗体
    参阅全部 Myelin oligodendrocyte glycoprotein 一抗
  • 描述
    山羊多克隆抗体to Myelin oligodendrocyte glycoprotein
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, WB, ELISA, IHC-P, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Horse, Cow, Pig
  • 免疫原

    Synthetic peptide:

    AGQFLEELRNPF

    with a Cysteine residue linker, corresponding to C terminal amino acids 236-247 of Human Myelin oligodendrocyte glycoprotein (NP_002424.3).

  • 阳性对照
    • Human brain cortex tissue; Human brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 28 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

靶标

图片

  • ab115597 staining Myelin oligodendrocyte glycoprotein in rat oligodendrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/150) for 10 hour at 4°C. An Alexa Fluor® 594-conjugated donkey anti-goat IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab115597, at 5µg/ml, staining Myelin oligodendrocyte glycoprotein in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Myelin oligodendrocyte glycoprotein antibody (ab115597) at 0.03 µg/ml + Human Brain lysate at 30 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa

文献

ab115597 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.01% Triton X100
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 18 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Rat Cell lysate - other (Oligodendrocytes)
Specification
Oligodendrocytes
Total protein in input
30 µg
Username

Abcam user community

Verified customer

提交于 Mar 12 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Oligodendrocytes)
Specification
Oligodendrocytes
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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