Anti-Myelin Basic Protein抗体[MBP101] (ab62631)

概述

  • 产品名称Anti-Myelin Basic Protein抗体[MBP101]
    参阅全部 Myelin Basic Protein 一抗
  • 描述
    小鼠单克隆抗体[MBP101] to Myelin Basic Protein
  • 经测试应用适用于: WB, ELISA, IHC-P, IHC-FoFr, ICC/IF, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Goat, Human
    预测可用于: Non Human Primates
  • 免疫原

    Purified human myelin basic protein.

性能

应用

Our Abpromise guarantee covers the use of ab62631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23700462
IHC-FoFr Use at an assay dependent concentration. PubMed: 24062649
ICC/IF 1/500.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 组织特异性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 疾病相关Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 序列相似性Belongs to the myelin basic protein family.
  • 发展阶段Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻译后修饰Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 细胞定位Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody [MBP101] 图像

  • ICC/IF image of Rat Oligodendrocytes primary culture stained with ab62631. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab62631, 2µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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  • ab62631 staining MBP in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with normal serum of species secondary antibody; antigen retrieval was by heat mediation in 10mM citric acid buffer. Samples were incubated with primary antibody (1/200) overnight at 4°C.

  • Overlay histogram showing SH-SY5Y cells stained with ab62631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Myelin Basic Protein antibody [MBP101] (ab62631)参考文献

This product has been referenced in:
  • Schmidt AF  et al. Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. J Neuroinflammation 13:238 (2016). IHC-P ; Rhesus monkey . Read more (PubMed: 27596440) »
  • Olympiou M  et al. Systemic inflammation disrupts oligodendrocyte gap junctions and induces ER stress in a model of CNS manifestations of X-linked Charcot-Marie-Tooth disease. Acta Neuropathol Commun 4:95 (2016). WB, IHC-Fr ; Mouse . Read more (PubMed: 27585976) »

See all 16 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Sciatic nerve
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Sciatic nerve
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Free-floating brain sections, 25 micron)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, HCL
Permeabilization Yes - 0.1% Triton-X100
Specification Free-floating brain sections, 25 micron
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Ms. Erika Villanueva

Verified customer

提交于 Nov 18 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton x-100 (in blocking step)
Fixative Paraformaldehyde
Username

Brandon Jun

Verified customer

提交于 Aug 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Sciatic nerve)
Loading amount 30 µg
Specification Sciatic nerve
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 May 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (intercostal nerve)
Specification intercostal nerve
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Gillian Hunter

Verified customer

提交于 Feb 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (sciatic nerve)
Loading amount 10 µg
Specification sciatic nerve
Gel Running Conditions Reduced Denaturing (8-12)
Blocking step No blocking step used for 30 minute(s) · Concentration: 100 · Temperature: 25°C
Username

Dr. Gillian Hunter

Verified customer

提交于 Sep 13 2012

Thank you for contacting us.

Myelin basic protein and myelin protein Zero are the common membrane associated proteins. Please check the datasheets

ab7349, ab40390, ab62631, ab53294 etc. and ab31851

ab128836 is an anti myelin ...

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Thank you for your call yesterday and for your patience.

I'm still waiting to hear back from thelab regardingthe light chain ofab66188, but I have been notified that ab62631 has akappa light chain.

I will be back in touch once I hav...

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As shown on the product datasheets (http://www.abcam.com/ab62631 and http://www.abcam.com/ab31851),those two antibodieshave never been tested in IHC-P and are not covered byour guarantee for this application.All tested applications are specified o...

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