Anti-Myelin Basic Protein抗体[BDI221] (ab66188)

概述

  • 产品名称Anti-Myelin Basic Protein抗体[BDI221]
    参阅全部 Myelin Basic Protein 一抗
  • 描述
    小鼠单克隆抗体[BDI221] to Myelin Basic Protein
  • 经测试应用适用于: Flow Cyt, IHC-FoFr, WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Goat, Human
    预测可用于: Non Human Primates
  • 免疫原

    Full length purified native protein (Human)

  • 表位Residues 130-136 of human myelin basic protein (21.5kDa and 18.5kDa molecular forms)

性能

应用

Our Abpromise guarantee covers the use of ab66188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21165693
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa.
Also suitable for quantitation of Myelin Basic Protein.
ELISA Use a concentration of 10 - 20 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml.
IHC-Fr Use a concentration of 3 - 5 µg/ml.

靶标

  • 功能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 组织特异性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 疾病相关Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 序列相似性Belongs to the myelin basic protein family.
  • 发展阶段Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻译后修饰Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 细胞定位Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody [BDI221] 图像

  • Overlay histogram showing PC12 cells stained with ab66188 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66188, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Myelin Basic Protein antibody [BDI221] (ab66188)参考文献

This product has been referenced in:
  • Yang Y  et al. Fibrinogen depleting agent batroxobin has a beneficial effect on experimental autoimmune encephalomyelitis. Cell Mol Neurobiol 31:437-48 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21165693) »

See 1 Publication for this product

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