Anti-Myelin Basic Protein抗体(ab40390)

概述

  • 产品名称Anti-Myelin Basic Protein抗体
    参阅全部 Myelin Basic Protein 一抗
  • 描述
    兔多克隆抗体to Myelin Basic Protein
  • 经测试应用适用于: ICC/IF, WB, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse Myelin Basic Protein.

    (Peptide available as ab40389.)

  • 阳性对照
    • This antibody gave a positive signal in the following Tissue Lysates: Brain (Mouse) and Brain (Rat).
  • 常规说明

    For a recombinant version of Myelin Basic Protein antibody (WB, ICC/IF, IHC-P, Human, Mouse, Rat) - see Ab209328 - clone IGX3421

性能

应用

Our Abpromise guarantee covers the use of ab40390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.Can be blocked with Mouse Myelin Basic Protein peptide (ab40389).
IHC-FoFr 1/1000.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 组织特异性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 疾病相关Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 序列相似性Belongs to the myelin basic protein family.
  • 发展阶段Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻译后修饰Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 细胞定位Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody 图像

  • All lanes : Anti-Myelin Basic Protein antibody (ab40390) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 18,23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
    This antibody was raised against an immunogen that is predicted to recognize isoforms (5, 7, 8, 10 and 13) of Myelin Basic Protein (MBP). The predicted molecular weights of isoforms (5, 7, 8, 10 and 13) are 18.5kDa, 17kDa, 14kDa, 21kDa and 13kDa respectively.
  • ICC/IF image of ab40390 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40390, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical detection of Myelin Basic Protein antibody using (ab40390) on PFA perfusion fixed free-floating rat brain sections. Primary antibody used at 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000).  Immunostaining is observed widely in tracts of axons, as expected. The pictures show the staining obtained at the level of the cerebral cortex, using the objective X5 (left) or X10 (right). The tissues were perfusion fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

  • ICC/IF image of ab40390 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40390, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab40390 staining rat adult brain saggital tissue section by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid (pH 6) prior to blocking in 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary.

  • ab40390 staining rat adult brain saggital tissue section by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid (pH 6) prior to blocking in 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary.

    See Abreview

Anti-Myelin Basic Protein antibody (ab40390)参考文献

This product has been referenced in:
  • Teo RT  et al. Structural and molecular myelination deficits occur prior to neuronal loss in the YAC128 and BACHD models of Huntington disease. Hum Mol Genet N/A:N/A (2016). WB ; Rat . Read more (PubMed: 27126634) »
  • Lazarus RC  et al. Protein Citrullination: A Proposed Mechanism for Pathology in Traumatic Brain Injury. Front Neurol 6:204 (2015). Rat . Read more (PubMed: 26441823) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Sensory neuronal co-culture)
Permeabilization Yes - 0.5% Triton
Specification Sensory neuronal co-culture
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 26 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections ( Mouse Tissue sections -Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization Yes - 0.1% Tween 20 in PBS
Specification  Mouse Tissue sections -Brain
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ms. Chia-Li Lin

Verified customer

提交于 Nov 23 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Horse Serum in 0.3% Triton X-100-PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Brandon Jun

Verified customer

提交于 Dec 17 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample Mouse Tissue sections (Spinal cord 16 micron cryosection)
Specification Spinal cord 16 micron cryosection
Permeabilization Yes - 0.5% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (prefrontal cortex)
Loading amount 50 µg
Specification prefrontal cortex
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain, prefrontal cortex)
Specification Brain, prefrontal cortex
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step None
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mei Deng

Verified customer

提交于 Sep 10 2012

Merci de nous avoir contactés.

Les deux anticorps sont garantis pour fonctionner en IHC (coupes en paraffine ou coupes congelées) et chez la souris.
Personnellement je recommanderais le polyclonal de lapin ab40390 car c'est u...

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Thank you for contacting us.

Myelin basic protein and myelin protein Zero are the common membrane associated proteins. Please check the datasheets

ab7349, ab40390, ab62631, ab53294 etc. and ab31851

ab128836 is an anti myelin ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Forebrain Tissue)
Loading amount 200 µg
Specification Forebrain Tissue
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Tom Hu

Verified customer

提交于 Apr 19 2012

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