Anti-Myelin Basic蛋白抗体(ab28541)
Key features and details
- Rabbit polyclonal to Myelin Basic Protein
- Suitable for: Sandwich ELISA
- Reacts with: Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Myelin Basic蛋白抗体
参阅全部 Myelin Basic Protein 一抗 -
描述
兔多克隆抗体to Myelin Basic蛋白 -
宿主
Rabbit -
特异性
This antibody is specific to myelin basic proteins. It may cross react with other isoforms of myelin basic proteins. -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Pig
预测可用于: Mammals -
免疫原
Full length native protein (purified) corresponding to Cow Myelin Basic Protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.00
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.01% Ammonium Acetate -
Concentration information loading...
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纯化说明
The antibody was affinity purified on agarose with an immuno dominant peptide (PRTPPPSQGKGRGL), which is a universal peptide sequence for all isoforms of mammalian myelin basic proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28541于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration. To be used as capture antibody. Pair with ab77895 - Rabbit polyclonal to Myelin Basic Protein (HRP)
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. To be used as capture antibody. Pair with ab77895 - Rabbit polyclonal to Myelin Basic Protein (HRP) |
靶标
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功能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
组织特异性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
疾病相关
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
序列相似性
Belongs to the myelin basic protein family. -
发展阶段
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻译后修饰
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
细胞定位
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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别名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
图片
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Fixed Immobilized Anti-MBP (ab28541): 2 ug/mL, 100 ul/well
Captured Antigen: Swine MBP , range from 1 to 90 ng/mL
Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt -
Immobilized Anti-MBP (ab28541): range from 0.125 to 2 µg/ml, 100 µl/well
Fixed Captured Antigen: Swine MBP, 500 ng/mL
Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab28541 被引用在 1 文献中.
- Li Y et al. Identification of Adrenomedullin-Induced S-Nitrosylated Proteins in JEG-3 Placental Cells. Reprod Sci N/A:N/A (2021). PubMed: 34462873