Anti-Myelin Basic Protein抗体[12] (ab92406)


  • 产品名称Anti-Myelin Basic Protein抗体[12]
    参阅全部 Myelin Basic Protein 一抗
  • 描述
    大鼠单克隆抗体[12] to Myelin Basic Protein
  • 特异性Myelin basic protein
  • 经测试应用适用于: ELISA, IHC-Fr, RIAmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide (Cow)

  • 表位ab92406 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87.



Our Abpromise guarantee covers the use of ab92406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • 组织特异性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • 疾病相关Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • 序列相似性Belongs to the myelin basic protein family.
    • 发展阶段Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • 翻译后修饰Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • 细胞定位Myelin membrane. Cytoplasmic side of myelin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GDB antibody
      • Golli MBP antibody
      • Golli MBP; myelin basic protein antibody
      • Hemopoietic MBP antibody
      • HMBPR antibody
      • HUGO antibody
      • MBP antibody
      • MBP_HUMAN antibody
      • MGC99675 antibody
      • MLD antibody
      • Myelin A1 protein antibody
      • Myelin basic protein antibody
      • Myelin Deficient antibody
      • Myelin membrane encephalitogenic protein antibody
      • OTTHUMP00000163776 antibody
      • OTTHUMP00000174387 antibody
      • OTTHUMP00000174388 antibody
      • SHI antibody
      • Shiverer antibody
      • SP antibody
      see all

    Anti-Myelin Basic Protein antibody [12] (ab92406)参考文献

    ab92406 has not yet been referenced specifically in any publications.

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    The antibody that would be suitable for the detection of the human myelin basic protein is the clone [12]. The epitope of this clone has 100% homology with the sequence you provided.
    The 2 Abcam references that...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Xenopus laevis Tissue sections (optic nerve)
    Specification optic nerve
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

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    提交于 May 03 2012

    I am happy to provide these codes for participation in our 100% Abreview discount program. We normally will not issue these retroactively but are happy to make an exception in this case for you. Should you wish to test further products please contact o...

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