MyD88 overexpression 293T裂解物(whole cell) (ab94067)
概述
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产品名称
MyD88 overexpression 293T裂解物(whole cell)
参阅全部 MyD88 细胞裂解液 -
常规说明
ab94067 is a 293T cell transfected lysate in which Human MyD88 has been transiently over-expressed using a pCMV-MyD88 plasmid. The lysate is provided in 1X Sample Buffer.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Tissue specificity: Ubiquitous. Disease: Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. Similarity: Contains 1 death domain. Contains 1 TIR domain. Domain: The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94067于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94067 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-MyD88 antibody (ab89576) at 1/500 dilution
Lane 1 :MyD88 overexpression 293T lysate (whole cell) (ab94067) at 25 µg
Lane 2 : 293T non-transfected lysate
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94067 尚未被引用在任何文献中。