概述

  • 产品名称Anti-MyD88抗体
    参阅全部 MyD88 一抗
  • 描述
    山羊多克隆抗体to MyD88
  • 经测试应用适用于: IHC-P, WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-IKYKAM KKEFP

    , corresponding to Internal sequence amino acids 255-265 of Human MyD88.

  • 阳性对照
    • Human T cell line MOLT4 cell lysate. Human thymus lysate. Human tonsil tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab28763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Sandwich ELISA Use a concentration of 5 - 10 µg/ml. Recombinant Antigen captured by rabbit antibody and ab28763 used as reporter

靶标

  • 功能Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 序列相似性Contains 1 death domain.
    Contains 1 TIR domain.
  • 结构域The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Anti-MyD88 antibody 图像

  • Anti-MyD88 antibody (ab28763) at 0.2 µg/ml + Human thymus lysate at 35 µg

    Predicted band size : 33 kDa
    Observed band size : 33 kDa

    Primary antibody incubated for 1 hour and detected using chemiluminescence.

  • Anti-MyD88 antibody (ab28763) at 0.01 µg/ml + Human T cell line MOLT4 cell lysate, 30µg total protein per lane

    Predicted band size : 33 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Primary incubation was for 1 hour.
    Detected using chemiluminescence.
  • ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Anti-MyD88 antibody (ab28763)参考文献

This product has been referenced in:
  • Szajnik M  et al. TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Oncogene 28:4353-63 (2009). IHC-P ; Human . Read more (PubMed: 19826413) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"