使用敲除细胞株进行验证

Anti-MyD88抗体[4D6] (ab107585)

概述

  • 产品名称Anti-MyD88抗体[4D6]
    参阅全部 MyD88 一抗
  • 描述
    小鼠单克隆抗体[4D6] to MyD88
  • 特异性Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).

  • 阳性对照
    • human ovary, human prostate and Jurkat cell lysates and CF-7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab107585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use 0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 序列相似性Contains 1 death domain.
    Contains 1 TIR domain.
  • 结构域The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Anti-MyD88 antibody [4D6] 图像



  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
    ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
  • Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
  • All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml

    Lane 1 : Human ovary cell lysate
    Lane 2 : Human prostate cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Jurkat cell lysate with Immunizing peptide

    Secondary
    Goat anti-mouse Ig HRP

    Predicted band size : 33 kDa

Anti-MyD88 antibody [4D6] (ab107585)参考文献

ab107585 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (B-cells)
Loading amount 30 µg
Specification B-cells
Treatment BL19LP overexpress MyD88
Gel Running Conditions Reduced Denaturing (10% SDS-Acrylamide gel 150V, 1h)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 08 2013

DISCOUNT CODE: ABTRIAL-0000
Ablaufdatum: 01 März. 2012

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