Anti-Mycobacterium tuberculosis Ag85B抗体(ab43019)

概述

  • 产品名称
    Anti-Mycobacterium tuberculosis Ag85B抗体
  • 描述
    兔多克隆抗体to Mycobacterium tuberculosis Ag85B
  • 特异性
    This antibody is specific for the Ag85B protein in sera, plasma or cell culture supernatant.
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    Reacts with Mycobacterium tuberculosis. Not yet tested in other species.
  • 免疫原

    Recombinant full length protein (Mycobacterium tuberculosis)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 0.05% Glycerol
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/3000 - 1/8000. Predicted molecular weight: 34 kDa.
ELISA 1/5000 - 1/10000.

靶标

  • 相关性
    Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall.
  • 别名
    • 30 kDa extracellular protein antibody
    • Antigen 85 complex B antibody
    • Mycobacterium antibody
    • Mycolyl transferase 85B antibody

图片

  • All lanes : Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) at 1/5000 dilution

    Lane 1 : E coli, expressing his-tagged Ag85b
    Lane 2 : E coli, expressing his-tagged Ag85b

    Secondary
    Lane 1 : HRP conjugated goat anti-rabbit IgG
    Lane 2 : HRP conjugated goat anti-rat IgG


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 28 kDa (possible non-specific binding,degradation product).

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab17976 staining Mycobacterium tuberculosis Ag85B in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 25°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Lin K  et al. Mycobacterium tuberculosis Thioredoxin Reductase Is Essential for Thiol Redox Homeostasis but Plays a Minor Role in Antioxidant Defense. PLoS Pathog 12:e1005675 (2016). WB . Read more (PubMed: 27249779) »
  • Torabi-Parizi P  et al. Pathogen-related differences in the abundance of presented antigen are reflected in CD4+ T cell dynamic behavior and effector function in the lung. J Immunol 192:1651-60 (2014). Read more (PubMed: 24431231) »

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Apr 10 2013

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

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Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

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Thank you for contacting us. Yes, the lab has confirmed that the immunogen for this antibody was His-tagged. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Purified protein (transformed E coli)
Specification
transformed E coli
Blocking step
fish gelatin as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type
Direct
Username

Abcam user community

Verified customer

提交于 Aug 29 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (Transformed E coli, expressing his-tagged Ag85b)
Loading amount
1.5 µg
Specification
Transformed E coli, expressing his-tagged Ag85b
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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