Anti-Mycobacterium tuberculosis Ag85抗体(ab36731)

概述

  • 产品名称
    Anti-Mycobacterium tuberculosis Ag85抗体
  • 描述
    小鼠单克隆抗体to Mycobacterium tuberculosis Ag85
  • 特异性
    This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
  • 免疫原

    Bacterial press extract from Mycobacterium tuberculosis H37Rv

  • 表位
    Epitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).
  • 常规说明

    WHO no. IT-49

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 骨髓瘤
    x63-Ag8.653
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab36731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
WB Use at an assay dependent dilution. Predicted molecular weight: 18,31,39 kDa.

靶标

  • 相关性
    Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
  • 别名
    • Antigen 85 complex A antibody
    • Antigen 85 complex B antibody
    • Antigen 85 complex C antibody
    • fbpA antibody
    • fbpB antibody
    • fbpC antibody
    • Secreted antigen 85 A antibody
    • Secreted antigen 85 B antibody
    • Secreted antigen 85 C antibody
    see all

实验方案

文献

This product has been referenced in:
  • Solans L  et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . Read more (PubMed: 24874799) »
  • Rengarajan J  et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . Read more (PubMed: 18172199) »

See all 4 Publications for this product

客户评价及客户问答



HYT 27, does react in WB with both Ag85A, B and C, see the paper by Armitige LY and colleagues, whom used HYT 27 in WB (on page 774, fbpA antibody) and showed very good reaction with A.
Paper:

Armitage et al., INFECTION AND IMM...

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Thank you for contacting us.



I have had the opportunity to speak with the lab regarding the specificity and cross reactivity of ab36731.

Specificity:

HYT 27 is specific for Mycobacterium mycolyltransferase. ...

Read More

Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (transformed E coli)
Loading amount
0.01 µg
Specification
transformed E coli
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

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Verified customer

提交于 Aug 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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