Anti-Mycobacterium tuberculosis Ag85抗体[HYT27] (ab36731)
Key features and details
- Mouse monoclonal [HYT27] to Mycobacterium tuberculosis Ag85
- Suitable for: ELISA, WB
- Isotype: IgG1
概述
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产品名称
Anti-Mycobacterium tuberculosis Ag85抗体[HYT27] -
描述
小鼠单克隆抗体[HYT27] to Mycobacterium tuberculosis Ag85 -
宿主
Mouse -
特异性
This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli. -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Mycobacterium tuberculosis
不与反应: Escherichia coli -
免疫原
Tissue, cells or virus corresponding to Mycobacterium tuberculosis Ag85.
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表位
Epitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid). -
常规说明
WHO no. IT-49
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
HYT27 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
1/400.
Cell culture filtrate or whole cells directly coated onto microtitre wells can be used. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 31,39 kDa.
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说明 |
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ELISA
1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 31,39 kDa. |
靶标
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相关性
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly. -
别名
- Antigen 85 complex A antibody
- Antigen 85 complex B antibody
- Antigen 85 complex C antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab36731 被引用在 6 文献中.
- Queval CJ et al. Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation. PLoS Pathog 17:e1009410 (2021). PubMed: 33720986
- Chai Q et al. A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy. Nat Commun 10:1973 (2019). PubMed: 31036822
- Solans L et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . PubMed: 24874799
- Rengarajan J et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . PubMed: 18172199
- Worsaae A et al. Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs. Infect Immun 55:2922-7 (1987). PubMed: 3119491
- Andersen AB et al. Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol 23:446-51 (1986). PubMed: 2420818