Anti-Mycobacterium L-alanine dehydrogenase抗体[WHO no. IT-46] (ab51804)

概述

  • 产品名称Anti-Mycobacterium L-alanine dehydrogenase抗体[WHO no. IT-46]
  • 描述
    小鼠单克隆抗体[WHO no. IT-46] to Mycobacterium L-alanine dehydrogenase
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    Reacts with Mycobacterium tuberculosis and M. marinum. Does not react with M. bovis BCG. Not yet tested in other species.
  • 免疫原

    Culture filtrate from Mycobacterium tuberculosis H37Rv

  • 表位Recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis.

性能

应用

Our Abpromise guarantee covers the use of ab51804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性Mycobacterium L-alanine dehydrogenase (ald) belongs to the AlaDH/PNT family and is a 40 kDa secreted protein from short term culture of Mycobacterium tuberculosis; which may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.
  • 细胞定位Secreted
  • 别名
    • 40 kDa antigen antibody
    • Alanine dehydrogenase antibody
    • ald antibody
    • TB43 antibody

Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] (ab51804)参考文献

This product has been referenced in:
  • Hutter B & Singh M Host vector system for high-level expression and purification of recombinant, enzymatically active alanine dehydrogenase of Mycobacterium tuberculosis. Gene 212:21-9 (1998). Read more (PubMed: 9661660) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mycobacterium tuberculosis Cell lysate - whole cell (Mycobacterium tuberculosis H37Rv)
Loading amount 40 µg
Specification Mycobacterium tuberculosis H37Rv
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Lay Harn Gam

Verified customer

提交于 May 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"