产品名称 Anti-MYCBP抗体 参阅全部 MYCBP 一抗
WB, ELISA, IHC-P, ICC/IF more details
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide: TPENPEIELL RLELAEMKEK YEAIVEENKK LKAKLAQYEP PQEEKRAE, corresponding to amino acids 56-103 of Human MYCBP
Jurkat cell lysate and human kidney tissue.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: JEG3.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 5 µg/ml.
功能 May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
组织特异性 Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.
序列相似性 Belongs to the AMY1 family.
细胞定位 Cytoplasm. Nucleus. Mitochondrion. Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.
Information by UniProt
AMY 1 antibody
Anti-MYCBP antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-MYCBP antibody (ab66331)
ICC/IF image of ab66331 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66331 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - MYCBP antibody (ab66331)
Anti-MYCBP antibody (ab66331) at 0.3 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 12 kDa Observed band size : 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MYCBP antibody (ab66331)
ab66331 at 4µg/ml staining MYCBP in epithelial cells of human kidney renal tubule (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MYCBP antibody(ab66331)
Immunohistochemistry of Human Intestine tissue at an antibody concentration of 4-8µg/ml using anti-MYCBP antibody (ab66331)
Anti-MYCBP antibody (ab66331)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"