Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide within Human MYCBP aa 54-103 (internal sequence). The exact sequence is proprietary. Numbering is based off mature sequence
Jurkat cell lysate and human kidney tissue.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: JEG3.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 5 µg/ml.
May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.
Belongs to the AMY1 family.
Cytoplasm. Nucleus. Mitochondrion. Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.
Information by UniProt
AMY 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-MYCBP antibody (ab66331)
ICC/IF image of ab66331 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66331 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - MYCBP antibody (ab66331)
Anti-MYCBP antibody (ab66331) at 0.3 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MYCBP antibody (ab66331)
ab66331 at 4µg/ml staining MYCBP in epithelial cells of human kidney renal tubule (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MYCBP antibody(ab66331)
Immunohistochemistry of Human Intestine tissue at an antibody concentration of 4-8µg/ml using anti-MYCBP antibody (ab66331)
has not yet been referenced specifically in any publications.
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