Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
c-myc tag antibody
Myc Epitope Tag antibody
Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)
All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
Lane 1 : HEK293 lysate - untransfected Lane 2 : HEK293 lysate - transfected with Myc-tagged protein
Immunocytochemistry/ Immunofluorescence - Myc tag antibody [Myc.A7] (ab18185)This image is courtesy of an anonymous Abreview
Top Panel: ab18185 staining Myc Tag in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-3% BSA) for 30 minutes at 20°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (1/500) was used as secondary antibody. Bottom panel: Synapses (Red); Anti-myc tag (Green)